Evidence map›Paper›PMID 39052221›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Automated Immunoprecipitation Workflow for Comprehensive Acetylome Analysis.

Marina A Gritsenko, Chia-Feng Tsai, Hyeyoon Kim, Tao Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Marina A GritsenkoBiological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
Chia-Feng TsaiBiological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
Hyeyoon KimBiological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA.
Tao LiuBiological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA. tao.liu@pnnl.gov.

Funding

Center for Advanced Multi-Omic Characterization of CancerU24CA271012 · NCI · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI Tao Liu · 2022 to 2026
$6.6M
PNNL Proteome Characterization CenterU24CA210955 · NCI · BATTELLE PACIFIC NORTHWEST LABORATORIES · PI LIU, TAO, SMITH, RICHARD D · 2016 to 2020
$5.3M
NCI NIH HHS U24 CA210955NCI NIH HHS U24 CA271012
6 · The paper itself

Abstract

Immunoprecipitation is one of the most effective methods for enrichment of lysine-acetylated peptides for comprehensive acetylome analysis using mass spectrometry. Manual acetyl peptide enrichment method using non-conjugated antibodies and agarose beads has been developed and applied in various studies. However, it is time-consuming and can introduce contaminants and variability that leads to potential sample loss and decreased sensitivity and robustness of the analysis. Here we describe a fast, automated enrichment protocol that enables reproducible and comprehensive acetylome analysis using a magnetic bead-based immunoprecipitation reagent.

Indexed as

ImmunoprecipitationWorkflowAcetylationHumansLysineMass SpectrometryPeptidesProtein Processing, Post-TranslationalProteomeProteomicsLysinePeptidesProteomeAcetylomeAcetyl peptide enrichmentAutomationImmunoprecipitationLysine acetylationMagnetic beadsMass spectrometryPost-translational modificationProteomics

Identifiers

PMID39052221
PMCPMC11949276

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.