Unbiased Quantitative Proteomics of Organoid Models of Pancreatic Cancer.

Ronnie Ren Jie Low, Ka Yee Fung, Laura F Dagley, Jumana Yousef, Samantha J Emery-Corbin, Tracy L Putoczki

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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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5 · Who and what money

Authors and funding

6 authors.

Ronnie Ren Jie LowThe Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.

Ka Yee FungThe Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.

Laura F DagleyThe Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.

Jumana YousefThe Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.

Samantha J Emery-CorbinThe Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.

Tracy L PutoczkiThe Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia. putoczki.t@wehi.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is a lethal solid malignancy with many patients succumbing to the disease within 6 months of diagnosis. The mechanisms that underlie PDAC initiation and progression are poorly understood. Current treatment options are primarily limited to chemotherapy, which is often provided with palliative intent. Unfortunately, there are no robust biomarkers to guide treatment selection or monitor treatment response. This is concerning given the increasing incidence of this cancer. We and others have generated organoid models to explore the biology underlying PDAC with the goal of identifying new therapeutic targets. Here we provide protocols to generate a preclinical PDAC organoid model and methods to use these to define the proteomic landscape of this cancer.

Indexed as

Carcinoma, Pancreatic DuctalOrganoidsPancreatic NeoplasmsProteomicsAnimalsBiomarkers, TumorHumansMiceProteomeBiomarkers, TumorProteomeFACsModelOrganoidPancreatic cancerProteomics

Identifiers

PMID39052215

DOI10.1038/s41467-022-35740-1

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.