ArticleFrontiers in microbiology2024
Establishment and application of quadruple fluorescence quantitative RT-PCR method for the identification of waterfowl astrovirus.
Article in Frontiers in microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Duplex MIRA assays for simultaneous detection of novel goose astrovirus and goose circovirus.BMC veterinary research · 2026Article
- Molecular evidence for goose astrovirus 2 and goose astrovirus 3 coinfection in goslings with gout.Archives of virology · 2025Article
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Authors and funding
11 authors.
Funding
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Abstract
Avian astrovirus can infect a variety of poultry species and cause viral diarrhea, with a wide epidemic range strong pathogenicity and a high incidence. Among them, Duck astrovirus 3(DAstV-3), Duck astrovirus 4(DAstV-4), Goose astrovirus 1(GoAstV-1) and Goose astrovirus 2(GoAstV-2) are four types of astroviruses newly discovered in waterfowl in recent years. In order to realize the rapid detection of these four kinds of waterfowl stellate viruses, specific primers and probes were engineered to target a highly conserved region of ORF1b gene of DAstV-3, GoAstV-1 and GoAstV-2 and the ORF2 gene of DAstV-4, and a quadruple fluorescence quantitative RT-PCR method was developed. The results indicate that the method established in this study has good specificity and no cross reactivity with other pathogens. This method can detect viruses with a minimum concentration of 1 × 10
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