Evidence map›Paper›PMID 39049649›Full record

ArticleHua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology2024

Role of the GRP78-c-Src signaling pathway on osteoblast differentiation of periodontal ligament fibroblasts induced by cyclic mechanical stretch.

Jing Hu, Zhihua Cui, Keqiang Huang, Rongjian Su, Song Zhao

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Article in Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jing HuPublic Experimental Platform, College of Life Science Institute, Jinzhou Medical University, Jinzhou 121001, China.
Zhihua CuiCenter of Oral Implantology, School and Hospital of Stomatology, China Medical University, Shenyang 110002, China.
Keqiang HuangDept. of Orthodontics, Affiliated Stomatological Hospital, Jinzhou Medical University, Jinzhou 121001, China.
Rongjian SuCollege of Basic Medical Science, Jinzhou Medical University, Jinzhou 121001, China.
Song ZhaoPublic Experimental Platform, College of Life Science Institute, Jinzhou Medical University, Jinzhou 121001, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesThis study aims to investigate the influence of glucose regulated protein (GRP) 78 on osteoblast differentiation in periodontal ligament fibroblasts (PDLFs) under cyclic mechanical stretch and determine the underlying mechanism.

methodsFlexCell 5000 cell mechanical device was applied to simulate the stress environment of orthodontic teeth. GRP78High and GRP78Low subpopulation were obtained by flow sorting. Gene transfection was performed to knockdown GRP78 and c-Src expression and overexpress c-Src. Western blot analysis was used to detect the protein expression of Runt-related gene 2 (RUNX2), Osterix, osteocalcin (OCN), and osteopontin (OPN). Immunoprecipitation assay was used to determine the interaction of GRP78 with c-Src. The formation of cellular mineralized nodules was determined by alizarin red staining.

resultsGRP78 was heterogeneously expressed in PDLFs, and GRP78High and GRP78Low subpopulations were obtained by flow sorting. The osteogenic differentiation ability and phosphorylation level of c-Src kinase in the GRP78High subpopulation were significantly increased compared with those in GRP78Low subpopulation after cyclic mechanical stretch (

conclusionsGRP78 upregulates c-Src expression by interacting with c-Src kinase and promotes osteogenic differentiation under cyclic mechanical stretch in PDLFs.

Indexed as

Cell DifferentiationHeat-Shock ProteinsOsteoblastsPeriodontal LigamentProto-Oncogene Proteins pp60(c-src)Signal TransductionStress, MechanicalCore Binding Factor Alpha 1 SubunitCSK Tyrosine-Protein KinaseEndoplasmic Reticulum Chaperone BiPFibroblastsHumansOsteocalcinOsteogenesisOsteopontinPhosphorylationCore Binding Factor Alpha 1 SubunitCSK Tyrosine-Protein KinaseEndoplasmic Reticulum Chaperone BiPHeat-Shock ProteinsHSPA5 protein, humanOsteocalcinOsteopontinProto-Oncogene Proteins pp60(c-src)src-Family Kinasescyclic mechanical stretchglucose regulated protein 78osteogenic differentiationperiodontal ligament fibroblasts

Identifiers

PMID39049649
PMCPMC11190863

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.