Evidence map›Paper›PMID 39046599›Full record

ArticleJournal of neuro-oncology2024

The CCL2-CCR4 axis promotes Regulatory T cell trafficking to canine glioma tissues.

W K Panek, R G Toedebusch, B E Mclaughlin, P J Dickinson, J E Van Dyke, K D Woolard, M E Berens, M S Lesniak, B K Sturges, K M Vernau and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuro-oncology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Review
  2. Article
  3. Immunotherapy in central nervous system tumors.International journal of surgery (London, England) · 2025
    Review
  4. Article
  5. Article
  6. Review
  7. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

W K PanekDepartment of Surgical and Radiological Sciences, University of California, Davis, One Shields Avenue, 2112 Tupper Hall, Davis, CA, 95616-5270, USA. wkp@upenn.edu.ORCID http://orcid.org/0000-0002-5667-5715
R G ToedebuschDepartment of Surgical and Radiological Sciences, University of California, Davis, One Shields Avenue, 2112 Tupper Hall, Davis, CA, 95616-5270, USA.
B E MclaughlinUniversity of California Davis, Flow Cytometry Shared Resource, Davis, CA, USA.ORCID http://orcid.org/0000-0001-5801-3996
P J DickinsonDepartment of Surgical and Radiological Sciences, University of California, Davis, One Shields Avenue, 2112 Tupper Hall, Davis, CA, 95616-5270, USA.ORCID http://orcid.org/0000-0002-0037-2619
J E Van DykeUniversity of California Davis, Flow Cytometry Shared Resource, Davis, CA, USA.
K D WoolardDepartment of Pathology, Microbiology and Immunology, University of California, Davis, Davis, CA, USA.ORCID http://orcid.org/0000-0003-3588-9359
M E BerensCancer and Cell Biology Division, The Translational Genomics Research Institute, Phoenix, AZ, USA.ORCID http://orcid.org/0000-0002-3382-6787
M S LesniakDepartment of Neurological Surgery, Lou and Jean Malnati Brain Tumor Institute, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.ORCID http://orcid.org/0000-0002-0096-5107
B K SturgesDepartment of Surgical and Radiological Sciences, University of California, Davis, One Shields Avenue, 2112 Tupper Hall, Davis, CA, 95616-5270, USA.
K M VernauDepartment of Surgical and Radiological Sciences, University of California, Davis, One Shields Avenue, 2112 Tupper Hall, Davis, CA, 95616-5270, USA.ORCID http://orcid.org/0000-0002-8062-0028
C LiDepartment of Surgical and Radiological Sciences, University of California, Davis, One Shields Avenue, 2112 Tupper Hall, Davis, CA, 95616-5270, USA.
J MiskaDepartment of Neurological Surgery, Lou and Jean Malnati Brain Tumor Institute, Robert H. Lurie Comprehensive Cancer Center, Feinberg School of Medicine, Northwestern University, Chicago, IL, USA.ORCID http://orcid.org/0000-0001-9608-2810
Christine M ToedebuschDepartment of Surgical and Radiological Sciences, University of California, Davis, One Shields Avenue, 2112 Tupper Hall, Davis, CA, 95616-5270, USA. cmtoedebusch@ucdavis.edu.ORCID http://orcid.org/0000-0002-3216-3344

Funding

Staff InvestigatorsP30CA093373 · NCI · UNIVERSITY OF CALIFORNIA DAVIS · PI KC KENT LLOYD · 2002 to 2026
$84.9M
UC Davis Paul Calabresi K12 Clinical Oncology Research Career Development ProgramK12CA138464 · NCI · UNIVERSITY OF CALIFORNIA AT DAVIS · PI BIRKELAND, ANDREW CHARLES, LARA, PRIMO N. · 2011 to 2025
$11.4M
UC Davis Shared Astrios Cell SorterS10OD018223 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI SHACKLETT, BARBARA L. · 2015 to 2015
$555k
NCI NIH HHS K12 CA138464NCI NIH HHS P30 CA093373NIH HHS S10 OD018223
6 · The paper itself

Abstract

purposeSpontaneously occurring glioma in pet dogs is increasingly recognized as a valuable translational model for human glioblastoma. Canine high-grade glioma and human glioblastomas share many molecular similarities, including the accumulation of immunosuppressive regulatory T cells (Tregs) that inhibit anti-tumor immune responses. Identifying in dog mechanisms responsible for Treg recruitment may afford to target the cellular population driving immunosuppression, the results providing a rationale for translational clinical studies in human patients. Our group has previously identified C-C motif chemokine 2 (CCL2) as a glioma-derived T-reg chemoattractant acting on chemokine receptor 4 (CCR4) in a murine orthotopic glioma model. Recently, we demonstrated a robust increase of CCL2 in the brain tissue of canine patients bearing high-grade glioma.

methodsWe performed a series of in vitro experiments using canine Tregs and patient-derived canine glioma cell lines (GSC 1110, GSC 0514, J3T-Bg, G06A) to interrogate the CCL2-CCR4 signaling axis in the canine.

resultsWe established a flow cytometry gating strategy for identifying and isolating FOXP3

conclusionOur study validates CCL2-CCR4 as a bi-directional Treg-glioma immunosuppressive and tumor-promoting axis in canine high-grade glioma.

Indexed as

Brain NeoplasmsChemokine CCL2GliomaReceptors, CCR4T-Lymphocytes, RegulatoryAnimalsCell Line, TumorCell MovementDogsHumansCCL2 protein, humanCCR4 protein, humanChemokine CCL2Receptors, CCR4CCL2CCR4DogGlioblastomaTumor-infiltrating lymphocyte

Identifiers

PMID39046599
PMCPMC11341612

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.