ArticleWorld journal of microbiology & biotechnology2024
Detection of vinblastine and related bioactive compounds from culture extracts of endophytic fungi of Catharanthus roseus.
Article in World journal of microbiology & biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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7 citing papers in PubMed.
- Profiling of Bioactive Secondary Metabolites of Colletotrichum siamense Isolated from Catharanthus roseus with Emphasis on Moschamine by Advanced Chromatographic and Mass Spectrometric Techniques.Current microbiology · 2026Article
- Novel production of vinblastine by immobilized cultures of mutant strain of the endophytic Alternaria alternata.World journal of microbiology & biotechnology · 2026Article
- Unveiling the diversity, bioactive metabolites and therapeutic potential of endophytic fungi of the Apocynaceae family: a comprehensive review.Archives of microbiology · 2026Review
- Endophytic fungus Muyocopron dipterocarpi isolated from Catharanthus roseus as a novel producer of ajmalicine and other metabolites: insights from HPLC and LC-HRMS analysis.Antonie van Leeuwenhoek · 2026Article
- Phyto-Fingerprinting of Putranjiva roxburghii Wall. Leaf Extract and its In Vitro Anti-Inflammatory Activity.Cell biochemistry and biophysics · 2025Article
- Sustainable vinblastine production by3 Biotech · 2025Article
- Nematicidal and Insecticidal Compounds from the Laurel Forest Endophytic FungusMolecules (Basel, Switzerland) · 2024Article
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2 authors.
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Abstract
This study investigates the synthesis of vinblastine by endophytic fungi isolated from leaf of C. roseus. A total of 10 endophytic fungi were selected for secretion of vinca alkaloids based on the initial screening by biochemical tests and thin-layer chromatography (TLC). Out of these ten, only four fungal extracts showed positive results for presence of vinblastine at same retention time (10 min.) compared to reference compound on HPLC analysis. The detected concentration of vinblastine was maximum (17 µg/ml) in isolate no. CRL 22 followed by CRL 52, CRL 17 and CRL 28. To validate the presence of vinblastine, ultra-high-performance liquid chromatography coupled with high-resolution accurate mass spectrometry (HRMS) was employed. This analysis confirmed the presence of anhydrovinblastine, a precursor of vinblastine through the detection of molecular ions at m/z 793.4185 in extract of CRL 17. In addition to anhydrovinblastine, the intermediate compounds essential to the biosynthetic pathway of vinblastine were also detected in the extract of CRL 17. These host-origin compounds strongly suggest the presence of a biosynthetic pathway within the endophytic fungus. Based on morphological observation and sequence analysis of the ITS region of rDNA, endophytic fungi were identified as Alternaria alternata (CRL 17), Curvularia lunata (CRL 28), Aspergillus terrus (CRL 52), and Aspergillus clavatonanicus (CRL 22).
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