Evidence map›Paper›PMID 39045008›Full record

ArticleFrontiers in cardiovascular medicine2024

Stem cell-derived cardiomyocytes expressing a dominant negative pacemaker HCN4 channel do not reduce the risk of graft-related arrhythmias.

Fanny Wulkan, Rocco Romagnuolo, Beiping Qiang, Tamilla Valdman Sadikov, Kyung-Phil Kim, Elya Quesnel, Wenlei Jiang, Naaz Andharia, Jill J Weyers, Nilesh R Ghugre and 3 more

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In one paragraph

Article in Frontiers in cardiovascular medicine, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Fanny Wulkan *McEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Rocco Romagnuolo *McEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Beiping QiangMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Tamilla Valdman SadikovMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Kyung-Phil KimBlueRock Therapeutics, Toronto, ON, Canada.
Elya QuesnelMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Wenlei JiangMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Naaz AndhariaMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Jill J WeyersPhysical Sciences Platform, Sunnybrook Research Institute, Toronto, ON, Canada.
Nilesh R GhugrePhysical Sciences Platform, Sunnybrook Research Institute, Toronto, ON, Canada.
Bilgehan OzcanMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Faisal J AlibhaiMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.
Michael A LaflammeMcEwen Stem Cell Institute, University Health Network, Toronto, ON, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) show tremendous promise for cardiac regeneration following myocardial infarction (MI), but their transplantation gives rise to transient ventricular tachycardia (VT) in large-animal MI models, representing a major hurdle to translation. Our group previously reported that these arrhythmias arise from a focal mechanism whereby graft tissue functions as an ectopic pacemaker; therefore, we hypothesized that hPSC-CMs engineered with a dominant negative form of the pacemaker ion channel HCN4 (dnHCN4) would exhibit reduced automaticity and arrhythmogenic risk following transplantation. Methods: We used CRISPR/Cas9-mediated gene-editing to create transgenic dnHCN4 hPSC-CMs, and their electrophysiological behavior was evaluated Results: Conclusions: While genetic silencing of the pacemaker ion channel HCN4 suppresses the automaticity of hPSC-CMs

Indexed as

cardiac regenerationcardiomyocytecell engineeringion channelmyocardial infarctionpacemaker currentpluripotent stem cellsventricular tachycardia

Identifiers

PMID39045008
PMCPMC11263024

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.