Evidence map›Paper›PMID 39044300›Full record

ArticleJournal of pharmaceutical health care and sciences2024

Eggshell membrane and green seaweed (Ulva lactuca) micronized powders for in vivo diabetic wound healing in albino rats: a comparative study.

Moustafa H Moustafa, Mohamed S Turkey, Noha S Mohamedin, Amira A Darwish, Amira A M Elshal, Mona A H Yehia, Mohamed M El Safwany, Ehab I Mohamed

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Article in Journal of pharmaceutical health care and sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Moustafa H MoustafaMedical Biophysics Department, Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, Alexandria, 21561, Egypt.ORCID https://orcid.org/0009-0008-1876-9041
Mohamed S TurkeyMicrobiology and Immunology Department, Faculty of Pharmacy, October 6 University, Sixth of October City, Giza, Egypt.ORCID https://orcid.org/0000-0002-1576-0841
Noha S MohamedinMedical Biophysics Department, Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, Alexandria, 21561, Egypt.ORCID https://orcid.org/0009-0008-2263-3745
Amira A DarwishMedical Laboratory Technology Department, Faculty of Applied Health Sciences Technology, Pharos University, Alexandria, Egypt.ORCID https://orcid.org/0000-0002-2607-5700
Amira A M ElshalMedical Biophysics Department, Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, Alexandria, 21561, Egypt.ORCID https://orcid.org/0009-0009-2883-4318
Mona A H YehiaHistochemistry and Cell Biology Department, Medical Research Institute, Alexandria University, Alexandria, Egypt.ORCID https://orcid.org/0000-0003-1046-4952
Mohamed M El SafwanyRadiological Sciences and Medical Imaging Department, Faculty of Applied Health Sciences Technology, Pharos University, Alexandria, Egypt.ORCID https://orcid.org/0000-0002-1186-4619
Ehab I MohamedMedical Biophysics Department, Medical Research Institute, Alexandria University, 165 El-Horreya Avenue, Alexandria, 21561, Egypt. ehab.abdo@alexu.edu.eg.ORCID http://orcid.org/0000-0002-4870-1928

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundNonhealing diabetic wounds are a serious complication associated with extremely lethargic wound closure and a high risk of infection, leading to amputation or limb loss, as well as substantial health care costs and a poor quality of life for the patient. The effects of either eggshell membrane (ESM) and green seaweed (Ulva lactuca) extracts alone or in combination were evaluated for in vivo skin wound healing in a rat model of induced diabetes.

methodsMicronized powders of waste hen ESM, Ulva lactuca, and their 1:1 mixture were prepared using regular procedures. The mechanical, electrical, and surface morphology characteristics of powders were examined using direct compression, LCR-impedancemetry, and scanning electron microscopy. The effect of ESM, Ulva lactuca, and their mixture as compared to standard Dermazin treatments were evaluated on wounds inflicted on male Wistar Albino rats with induced diabetes. Quantitative wound healing rates at baseline and at 3, 7, 14, and 21 days of treatments among all rat groups were conducted using ANOVA. Qualitative histological analysis of epidermal re-epithelization, keratinocytes, basement membrane, infiltrating lymphocytes, collagen fibrines, and blood vessels at day 21 were performed using Image J processing program.

resultsCompressive strength measurements of tablets showed a Young's modulus of 44.14 and 27.17 MPa for the ESM and ESM + Ulva lactuca mixture, respectively. Moreover, both samples exhibited relatively low relative permittivity values of 6.62 and 6.95 at 1 MHz, respectively, due to the porous surface morphology of ESM shown by scanning electron microscopy. On day 21, rats treated with ESM had a complete diabetic wound closure, hair regrowth, and a healing rate of 99.49%, compared to 96.79% for Dermazin, 87.05% for Ulva lactuca, 90.23% for the mixture, and only 36.44% for the negative controls. A well-formed basement membrane, well-differentiated epithelial cells, and regular thick keratinocytes lining the surface of the epidermal cells accompanied wound healing in rats treated with ESM, which was significantly better than in control rats.

conclusionGround hen ESM powder, a low-cost effective biomaterial, is better than Ulva lactuca or their mixture for preventing tissue damage and promoting diabetic wound healing, in addition to various biomedical applications.

Indexed as

Compressive strengthDielectric constantEggshell membrane (ESM)Induced-diabetes woundSeaweed (Ulva lactuca)Wound healing

Identifiers

PMID39044300
PMCPMC11265442

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.