Evidence map›Paper›PMID 39044090›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Ex Vivo Fluorescent Labeling of HBV-Specific B Cells in Chronic Hepatitis B Patients.

Loghman Salimzadeh, Alice R Burton, Nina Le Bert

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Loghman SalimzadehSchwartz-Reisman Liver Research Centre, University Health Network, Toronto, ON, Canada. loghman.salimzadeh@uhn.ca.
Alice R BurtonDivision of Infection and Immunity, Institute of Immunity and Transplantation, University College London, London, UK.
Nina Le BertEmerging Infectious Diseases Program, Duke-NUS Medical School, Singapore, Singapore.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescently conjugated antigen-bait systems have been extensively used to identify antigen-specific B cells and probe humoral immunity across different settings. Following this approach, we used HBV antigens to bind the B cell receptor (BCR), permitting antigen-specific B cell detection by flow cytometry. Fluorochromes can either be attached covalently via chemical conjugation to the antigen or attached non-covalently by biotinylating the antigen. Dual-staining antigen-baits (where an antigen is directly conjugated to two distinct fluorochromes) have now been used to identify HBsAg- and HBcAg-specific B cells with a high degree of reliability and specificity. This system can be used to detect and characterize cells ex vivo or adapted to isolate antigen-specific cells using fluorescence-activated cell sorting.

Indexed as

B-LymphocytesFlow CytometryFluorescent DyesHepatitis B, ChronicHepatitis B Surface AntigensHepatitis B virusHepatitis B Core AntigensHumansReceptors, Antigen, B-CellStaining and LabelingFluorescent DyesHepatitis B Core AntigensHepatitis B Surface AntigensReceptors, Antigen, B-CellAnti-HBsAnti-HBV specific B cellsELISpotFlow cytometryFluorochrome conjugated baits

Identifiers

PMID39044090

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.