Evidence map›Paper›PMID 39044088›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Peptide-Loaded HLA Class I Tetramer-Associated Magnetic Bead-Based Enrichment of HBV-Specific CD8+ T Cells.

Kathrin Heim, Maike Hofmann, Robert Thimme

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Kathrin HeimDepartment of Medicine II, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Maike HofmannDepartment of Medicine II, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Robert ThimmeDepartment of Medicine II, Medical Center, Faculty of Medicine, University of Freiburg, Freiburg, Germany. robert.thimme@uniklinik-freiburg.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

HBV-specific CD8+ T cells are only present at the low frequency during chronic infection. Thus, they are often undetectable by conventional ex vivo staining methods using peptide-loaded HLA class I tetramers. Detection sensitivity can be increased by magnetic bead-based enrichment strategies following staining with peptide-loaded HLA class I tetramers. Additionally, some downstream applications like e.g., single cell RNA sequencing of virus-specific CD8+ T cells may also require a pre-enrichment step to increase the frequency of the cells of interest. For this, peptide-loaded HLA class I tetramers-associated magnetic bead-based enrichment is also a suitable method.

Indexed as

CD8-Positive T-LymphocytesHepatitis B virusHistocompatibility Antigens Class IPeptidesEpitopes, T-LymphocyteHepatitis BHumansImmunomagnetic SeparationEpitopes, T-LymphocyteHistocompatibility Antigens Class IPeptidesEnrichment strategyHBVHLA class IPeptide-loaded HLA class I tetramersVirus-specific CD8+ T cells

Identifiers

PMID39044088

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.