A Chromogenic In Situ Hybridization (CISH) Assay for Detection of HBV RNA, DNA, and cccDNA in Liver Tissue.

Xiaonan Zhang, Wei Lu, Yanling Feng, Zhanqing Zhang, Zhenghong Yuan

Abstract read

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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors.

Xiaonan ZhangCentre for Research in Therapeutic Solutions, Biomedical Sciences, Faculty of Science and Technology, University of Canberra, Canberra, ACT, Australia. Xiaonan.Zhang@canberra.edu.au.

Wei LuShanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Yanling FengShanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Zhanqing ZhangShanghai Public Health Clinical Center, Fudan University, Shanghai, China.

Zhenghong YuanKey Laboratory of Medical Molecular Virology (MOE & NHC), Research Unit of Cure of Chronic Hepatitis B Virus Infection (CAMS), Shanghai Frontiers Science Center of Pathogenic Microorganisms and Infection, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai, China. zhyuan@shmu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Hepatitis B virus (HBV) developed highly intricates mechanisms exploiting host resources for its multiplication within a constrained genetic coding capacity. With the aid of a series of classical analytical methods such as ultrafiltration, and Southern and Northern blots, a general framework of HBV life cycle has been established. However, this picture still lacks many key histological contexts which involves pathophysiological changes of hepatocytes, non-parenchymal cells, infiltrated leukocytes, and associated extracellular matrix. Here, we describe a CISH protocol modified from the ViewRNA assay that allows direct visualization of HBV RNA, DNA, and cccDNA in liver tissue of chronic hepatitis B patients. By coupling it with immunohistochemistry and other histological stains, much richer information regarding the HBV-induced pathological changes can be harvested.

Indexed as

DNA, ViralHepatitis B virusIn Situ HybridizationLiverRNA, ViralChromogenic CompoundsDNA, CircularHepatitis B, ChronicHumansImmunohistochemistryChromogenic CompoundsDNA, CircularDNA, ViralRNA, ViralcccDNACISHHBVImmunohistochemistry

Identifiers

PMID39044081

DOI10.1172/jci83339

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.