Evidence map›Paper›PMID 39043820›Full record

ArticleScientific reports2024

Novel lytic bacteriophage AhFM11 as an effective therapy against hypervirulent Aeromonas hydrophila.

Nithin Muliya Sankappa, Girisha Shivani Kallappa, Kushala Kallihosuru Boregowda, Namrutha Mandrira Ramakrishna, Prithvisagar Kattapuni Suresh, Dheeraj Shriraje Balakrishna, Krishna Kumar Ballamoole, Suresh Thangavel, Lopamudra Sahoo, Miles D Lange and 2 more

Abstract read
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Bacteriophages AgainstViruses · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Nithin Muliya Sankappa *Department of Aquatic Animal Health Management, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangaluru, Karnataka, 575002, India.
Girisha Shivani Kallappa *Department of Aquatic Animal Health Management, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangaluru, Karnataka, 575002, India. skgirisha@gmail.com.
Kushala Kallihosuru Boregowda *Department of Aquatic Animal Health Management, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangaluru, Karnataka, 575002, India.
Namrutha Mandrira Ramakrishna *Department of Aquatic Animal Health Management, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangaluru, Karnataka, 575002, India.
Prithvisagar Kattapuni SureshDivision of Infectious Diseases, Nitte University Centre for Science Education and Research, Mangaluru, India.
Dheeraj Shriraje BalakrishnaDepartment of Aquatic Animal Health Management, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangaluru, Karnataka, 575002, India.
Krishna Kumar BallamooleDivision of Infectious Diseases, Nitte University Centre for Science Education and Research, Mangaluru, India.
Suresh ThangavelDepartment of Aquatic Animal Health Management, College of Fisheries, Karnataka Veterinary, Animal and Fisheries Sciences University, Matsyanagar, Mangaluru, Karnataka, 575002, India.
Lopamudra SahooDepartment of Fish Genetics and Reproduction, College of Fisheries, Central Agricultural University (Imphal), Lembucherra, Tripura West, Tripura, 799210, India.
Miles D LangeAquatic Animal Health Research Unit, United States Department of Agriculture, Agricultural Research Service, Auburn, AL, 36832, USA.
Michael B DeshotelUnited States Department of Agriculture, Agricultural Research Service, Harry K. Dupree Stuttgart National Aquaculture Research Center, Stuttgart, AR, 72160, USA.
Jason W AbernathyAquatic Animal Health Research Unit, United States Department of Agriculture, Agricultural Research Service, Auburn, AL, 36832, USA. jason.abernathy@usda.gov.ORCID 0000-0001-6084-8239

Funding

Department of Biotechnology , Ministry of Science and Technology (DBT) BT/PR17014/NER/95/390/2015United States Department of Agriculture | Agricultural Research Service (USDA Agricultural Research Service) 6010-32000-027-000-DUnited States Department of Agriculture | Agricultural Research Service (USDA Agricultural Research Service) 6028-31630-009-000-D
6 · The paper itself

Abstract

Several farmed fish species, including carps, tilapia, salmon, and catfish, have experienced significant economic losses in aquaculture due to motile Aeromonas septicemia caused by Aeromonas hydrophila. In the present study, a novel lytic bacteriophage infecting hypervirulent Aeromonas hydrophila (vAh) was isolated and characterized. This is the first report of a phage against vAh. Phage AhFM11 demonstrated lytic activity against both vAh strains and the A. hydrophila reference strain ATCC 35654. The AhFM11 genome was sequenced and assembled, comprising 168,243 bp with an average G + C content of 41.5%. The genome did not harbor any antibiotic resistance genes. Genomic information along with transmission electron microscopy revealed that phage AhFM11 belongs to the Straboviridae family. Therapeutic application of monophage AhFM11 in fish showed 100% survival in injection, 95% in immersion and 93% in oral feeding of phage top-coated feed. Fish and chicken meat spiked with A. hydrophila and phage showed significant reduction of A. hydrophila. These findings support that phage AhFM11 can be used as a biocontrol agent against vAh as an alternative to antibiotics.

Indexed as

Aeromonas hydrophilaBacteriophagesGram-Negative Bacterial InfectionsAnimalsFish DiseasesFishesGenome, ViralPhage TherapyVirulenceAntibiotic resistance genes and biocontrolAquacultureBacteriophage therapyHypervirulent Aeromonas hydrophilaLytic phage

Identifiers

PMID39043820
PMCPMC11266544

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.