ArticleNature communications2024
4D Single-particle tracking with asynchronous read-out single-photon avalanche diode array detector.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Sequence-defined polymers for predictable gene delivery.Nature reviews. Chemistry · 2026Review
- Review of Machine Learning for Single-Particle Tracking: Methods, Challenges, and Biophysical Insights.Chemical & biomedical imaging · 2026Review
- 3D Single-Virus Tracking: Advances in Methodology and Labeling Strategies Towards Probing the Virus-Epithelium Interaction.Viruses · 2026Review
- Initial Distance-Dependent Mean Force Drives Synaptic Vesicle Motion Toward Fusion Sites in Stimulated Hippocampal Neurons.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Perspective: An outlook on fluorescence tracking.The Journal of chemical physics · 2025Article
- Single-Photon Single-Particle Tracking.bioRxiv : the preprint server for biology · 2025Article
- Super-Resolving Particle Diffusion Heterogeneity in Porous Hydrogels via High-Speed 3D Active-Feedback Single-Particle Tracking Microscopy.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Single molecule spectrum dynamics imaging with 3D target-locking tracking.Nature communications · 2025Article
- Localization of single molecules with structured illumination and structured detection.Light, science & applications · 2025Article
- Wide-field fluorescence lifetime imaging of single molecules with a gated single-photon camera.Light, science & applications · 2025Article
- Super-resolving particle diffusion heterogeneity in porous hydrogels via high-speed 3D active-feedback single-particle tracking microscopy.bioRxiv : the preprint server for biology · 2025Article
- Array detection enables large localization range for simple and robust MINFLUX.Light, science & applications · 2025Article
- Wavefront estimation through structured detection in laser scanning microscopy.Biomedical optics express · 2025Article
- Spatiotemporal Exciton Tracking with a SPAD Camera.ACS photonics · 2025Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Single-particle tracking techniques enable investigation of the complex functions and interactions of individual particles in biological environments. Many such techniques exist, each demonstrating trade-offs between spatiotemporal resolution, spatial and temporal range, technical complexity, and information content. To mitigate these trade-offs, we enhanced a confocal laser scanning microscope with an asynchronous read-out single-photon avalanche diode array detector. This detector provides an image of the particle's emission, precisely reflecting its position within the excitation volume. This localization is utilized in a real-time feedback system to drive the microscope scanning mechanism and ensure the particle remains centered inside the excitation volume. As each pixel is an independent single-photon detector, single-particle tracking is combined with fluorescence lifetime measurement. Our system achieves 40 nm lateral and 60 nm axial localization precision with 100 photons and sub-millisecond temporal sampling for real-time tracking. Offline tracking can refine this precision to the microsecond scale. We validated the system's spatiotemporal resolution by tracking fluorescent beads with diffusion coefficients up to 10 μm
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.