Generation of tools for expression and purification of the phage-encoded Type I restriction enzyme inhibitor, Ocr.

Joana Alves, Inga Dry, John H White, David T F Dryden, Nicola N Lynskey

Abstract read

In one paragraph

Article in Microbiology (Reading, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

Acquisition of a novel restriction modification system regulates genetic flux and gene expression in the hypervirulent and globally disseminated CC17 lineage of group B Streptococcus.Nucleic acids research · 2026
Article
BlihIA-A Novel Type I Restriction-Modification System fromInternational journal of molecular sciences · 2025
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Joana AlvesThe Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, Scotland, EH25 9RG, UK.

Inga DryThe Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, Scotland, EH25 9RG, UK.

John H WhiteEaStCHEM School of Chemistry, University of Edinburgh, The King's Buildings, Edinburgh, EH9 3FJ, UK.

David T F DrydenDepartment of Biosciences, University of Durham, South Road, DH1 3LE, UK.

Nicola N LynskeyThe Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, Scotland, EH25 9RG, UK.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

DNA manipulation is an essential tool in molecular microbiology research that is dependent on the ability of bacteria to take up and preserve foreign DNA by horizontal gene transfer. This process can be significantly impaired by the activity of bacterial restriction modification systems; bacterial operons comprising paired enzymatic activities that protectively methylate host DNA, while cleaving incoming unmodified foreign DNA. Ocr is a phage-encoded protein that inhibits Type I restriction modification systems, the addition of which significantly improves bacterial transformation efficiency. We recently established an improved and highly efficient transformation protocol for the important human pathogen group A

Indexed as

Recombinant ProteinsBacteriophagesDeoxyribonucleases, Type I Site-SpecificEscherichia coliGene ExpressionStreptococcus pyogenesTransformation, BacterialViral ProteinsDeoxyribonucleases, Type I Site-SpecificRecombinant ProteinsViral Proteins

Identifiers

PMID39042422

DOI10.1099/mic.0.001465

PMCPMC11317969

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.