Evidence map›Paper›PMID 39042282›Full record

ArticleMolecular biology reports2024

Expression mechanisms of mir-486-5p and its host gene sANK1 in porcine muscle.

Xiaoping Guo, Ganqiu Lan, Qinyang Jiang, Yafen Guo, Yiqiang Ouyang, Jing Liang, Mingyuan Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Xiaoping GuoLaboratory Animal Center, Guangxi Medical University, Nanning, 530021, Guangxi, China.
Ganqiu LanCollege of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China.
Qinyang JiangCollege of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China.
Yafen GuoCollege of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China.
Yiqiang OuyangLaboratory Animal Center, Guangxi Medical University, Nanning, 530021, Guangxi, China.
Jing LiangCollege of Animal Science and Technology, Guangxi University, Nanning, 530004, Guangxi, China. liangjing@gxu.edu.cn.
Mingyuan ZhangLaboratory Animal Center, Guangxi Medical University, Nanning, 530021, Guangxi, China. Bio_MYZhang@sr.gxmu.edu.cn.

Funding

National Modern Agricultural Industrial Technological System in Guangxi pig industry innova-tion team project nycytxgxcxtd-03-15National Natural Science Foundation of China 30960058
6 · The paper itself

Abstract

backgroundMiR-486-5p has been identified as a crucial regulator of the PI3K/AKT signalling pathway, which plays a significant role in skeletal muscle development. Its host gene, sANK1, is also essential for skeletal muscle development. However, the understanding of porcine miR-486-5p and sANK1 has been limited. METHODS AND

resultsIn this study, PCR analyses revealed a positive correlation between the expression of miR-486-5p and sANK1 in the longissimus dorsi muscle of the Bama mini-pig and Landrace-pig, as well as during myoblast differentiation. Furthermore, the expression of miR-486-5p/sANK1 was higher in the Bama mini-pig compared to the Landrace-pig. There was a total of 18 single nucleotide polymorphisms (SNP) present in the sANK1 promoter region. Among these SNPs, 14 of them resulted in alterations in transcription factor binding sites (TFBs). Additionally, the promoter fluorescence assay demonstrated that the activity of the sANK1 promoter derived from the Bama mini-pig was significantly higher compared to Landrace-pig. It is worth noting that ten regulatory SNPs have the potential to influence the activity of the sANK1 promoter. A nuclear mutation A-G located at position - 401 (relative to the transcription start site) in the Bama mini-pig was identified, which creates a putative TFB motif for MyoD.

conclusionsThe findings presented in this study offer fundamental molecular knowledge and expression patterns of miR-486-5p/sANK1, which can be valuable for gaining a deeper understanding of the gene's involvement in porcine skeletal muscle development, and meat quality.

Indexed as

MicroRNAsMuscle, SkeletalPolymorphism, Single NucleotidePromoter Regions, GeneticAnimalsBinding SitesCell DifferentiationGene Expression RegulationMuscle DevelopmentMyoblastsMyoD ProteinSignal TransductionSwineMicroRNAsMyoD ProteinmiR-486-5pPigsANK1Skeletal muscle

Identifiers

PMID39042282

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.