Evidence map›Paper›PMID 39039427›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

3D Spheroid and Organoid Models to Study Neuroinfection of RNA Viruses.

Vinicius Pinho Dos Reis, Karsten Cirksena, Agnieszka Rybak-Wolf, Bettina Seeger, Eva Herker, Gisa Gerold

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Vinicius Pinho Dos ReisInstitute of Virology, Philipps-University Marburg, Marburg, Germany.
Karsten CirksenaDepartment of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany.
Agnieszka Rybak-WolfOrganoid Platform, Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Association, Berlin, Germany. agnieszka.rybak@mdc-berlin.de.
Bettina SeegerInstitute for Food Quality and Safety, University of Veterinary Medicine Hannover, Hannover, Germany. bettina.seeger@tiho-hannover.de.
Eva HerkerInstitute of Virology, Philipps-University Marburg, Marburg, Germany. eva.herker@uni-marburg.de.
Gisa GeroldDepartment of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany. Gisa.Gerold@tiho-hannover.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Three-dimensional culture models of the brain enable the study of neuroinfection in the context of a complex interconnected cell matrix. Depending on the differentiation status of the neural cells, two models exist: 3D spheroids also called neurospheres and cerebral organoids. Here, we describe the preparation of 3D spheroids and cerebral organoids and give an outlook on their usage to study Rift Valley fever virus and other neurotropic viruses.

Indexed as

OrganoidsSpheroids, CellularAnimalsBrainCell Culture TechniquesCell Culture Techniques, Three DimensionalHumansRNA VirusesRNA Virus Infections3D spheroidCerebral organoidInduced pluripotent stem cellsNeural progenitor cellsNeuroinfectionNeurosphereNeurotropic virus

Identifiers

PMID39039427

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.