Characterization of RVFV Nucleocapsid Protein Binding Sites on RNA by iCLIP-seq.

Miyuki Hayashi, J Stephen Lodmell

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Miyuki HayashiDepartment of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA, USA.

J Stephen LodmellDepartment of Chemistry and Biochemistry, University of Montana, Missoula, MT, USA. stephen.lodmell@umontana.edu.

Funding

Surveillance genome sequencing to detect SARS-CoV-2 virus variants in MontanaP30GM140963 · NIGMS · UNIVERSITY OF MONTANA · PI BOWLER, BRUCE E · 2021 to 2025

$6.9M

A role for snoRNAs in RVFV-induced enhancement of alternative splicingR03AI137620 · NIAID · UNIVERSITY OF MONTANA · PI LODMELL, J. STEPHEN · 2019 to 2020

$145k

NIAID NIH HHS R03 AI137620NIGMS NIH HHS P30 GM140963

6 · The paper itself

Abstract

The nucleocapsid protein (N) in Rift Valley fever virus is an RNA-binding protein that functions in viral transcription, replication, and packaging. In this chapter, the method for studying protein-RNA interactions in context of viral infection using individual nucleotide resolution, cross-linking, immunoprecipitation, and sequencing (iCLIP-seq) is explained. The method is useful for identifying the interactions between both host and viral RNAs with N and can identify RNA motifs that interact with the protein of interest.

Indexed as

ImmunoprecipitationNucleocapsid ProteinsRift Valley fever virusRNA, ViralBinding SitesHigh-Throughput Nucleotide SequencingHumansProtein BindingRNA-Binding ProteinsNucleocapsid ProteinsRNA-Binding ProteinsRNA, ViraliCLIPNucleocapsid proteinRift Valley fever virusRNA

Identifiers

PMID39039420

DOI10.1007/978-1-0716-3926-9_19

PMCPMC13246016

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.