Evidence map›Paper›PMID 39039287›Full record

ArticleThe EMBO journal2024

CMG helicase disassembly is essential and driven by two pathways in budding yeast.

Cristian Polo Rivera, Tom D Deegan, Karim P M Labib

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The EMBO journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Ubiquitin and SUMO pathways in DNA replication and replication-coupled repair.Critical reviews in biochemistry and molecular biology
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Cristian Polo RiveraMRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK.ORCID http://orcid.org/0000-0002-1433-4111
Tom D DeeganMRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK. t.deegan@ed.ac.uk.ORCID http://orcid.org/0000-0001-9639-7636
Karim P M LabibMRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dundee, DD1 5EH, UK. kpmlabib@dundee.ac.uk.ORCID http://orcid.org/0000-0001-8861-379X

Funding

Cancer Research UK 24558Cancer Research UK DRCRPG-NOV22/100016Cancer Research UK (CRUK) C578/A24558Cancer Research UK (CRUK) C578/A25669UKRI | Medical Research Council (MRC) MC_UU_0035/4UKRI | Medical Research Council (MRC) MC_UU_12016/13
6 · The paper itself

Abstract

The CMG helicase is the stable core of the eukaryotic replisome and is ubiquitylated and disassembled during DNA replication termination. Fungi and animals use different enzymes to ubiquitylate the Mcm7 subunit of CMG, suggesting that CMG ubiquitylation arose repeatedly during eukaryotic evolution. Until now, it was unclear whether cells also have ubiquitin-independent pathways for helicase disassembly and whether CMG disassembly is essential for cell viability. Using reconstituted assays with budding yeast CMG, we generated the mcm7-10R allele that compromises ubiquitylation by SCF

Indexed as

DNA HelicasesSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsCell Cycle ProteinsDNA ReplicationF-Box ProteinsMinichromosome Maintenance Complex Component 7Minichromosome Maintenance ProteinsUbiquitinationCell Cycle ProteinsDia2 protein, S cerevisiaeDNA HelicasesF-Box ProteinsMCM10 protein, S cerevisiaeMCM7 protein, S cerevisiaeMinichromosome Maintenance Complex Component 7Minichromosome Maintenance ProteinsPIF1 protein, S cerevisiaeRrm3 protein, S cerevisiaeSaccharomyces cerevisiae ProteinsCMG HelicaseDNA ReplicationPif1-Rrm3SCFDia2Ubiquitylation

Identifiers

PMID39039287
PMCPMC11405719

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.