ArticleScientific reports2024
Optimised human stool sample collection for multi-omic microbiota analysis.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed.
- Microbiota-Targeted Chitooligosaccharides Intervention Restores Glucose Homeostasis After Islet Cell Transplantation in Rapamycin-Treated Mice.Food science & nutrition · 2026Article
- Bacteroides-derived endocannabinoid-like commendamide attenuates skeletal muscle ferroptosis in vitro: implications for Duchenne muscular dystrophy.Journal of translational medicine · 2026Article
- S'Wipe: user-friendly stool collection for high-throughput gut metabolomics and multi-omics.mSystems · 2026Article
- Gut microbial signatures and stability are associated with a co-diagnosis of endometriosis and inflammatory bowel disease.iScience · 2026Article
- The gut microbiome as a modifiable contributor to autism spectrum disorder: a precision gut-immune-brain perspective.Frontiers in child and adolescent psychiatry · 2026Article
- Gut microbiota and intestinal permeability in rheumatoid arthritis: pathogenic mechanisms.Frontiers in immunology · 2026Review
- The gut microbiome-cardiometabolic axis: insights into obesity, type 2 diabetes, and hypertension.Frontiers in endocrinology · 2026Review
- Review
- The Athlete Gut Microbiome: A Narrative Review of Multi-Omics Insights and Next-Generation Probiotic Strategies.Nutrients · 2025Review
- Microbiome impacts surgical outcomes- comparison of gut microbiome dysbiosis in patients undergoing lumbar microdiscectomy: results from the MISO pilot study.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2025Article
- OMNImet®•GUT collection kit supports sample collection and ambient temperature preservation of faecal short chain fatty acids.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- Microbiome in heritage: how maternal microbiome transmission impacts next generation health.Microbiome · 2025Review
- Comparative study of sample storage conditions on gut dysbiosis in peripheral artery disease.Research square · 2025Article
- Evaluating long-term stool preservation methods for maximizing the recovery of viable human fecal microbiota.Gut microbes reports · 2025Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
To accurately define the role of the gut microbiota in health and disease pathogenesis, the preservation of stool sample integrity, in terms of microbial community composition and metabolic function, is critical. This presents a challenge for any studies which rely on participants self-collecting and returning stool samples as this introduces variability and uncertainty of sample storage/handling. Here, we tested the performance of three stool sample collection/preservation buffers when storing human stool samples at different temperatures (room temperature [20 °C], 4 °C and - 80 °C) for up to three days. We compared and quantified differences in 16S rRNA sequencing composition and short-chain fatty acid profiles compared against immediately snap-frozen stool. We found that the choice of preservation buffer had the largest effect on the resulting microbial community and metabolomic profiles. Collectively analysis confirmed that PSP and RNAlater buffered samples most closely recapitulated the microbial diversity profile of the original (immediately - 80 °C frozen) sample and should be prioritised for human stool microbiome studies.
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Registered trials
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