Reversible Conjugation of Polypeptides and Proteins Utilizing a [3.3.1] Scaffold under Mild Conditions.

Ryan J Bartlett, Kelly D Crisostomo, Qiang Zhang

Abstract read

In one paragraph

Article in Organic letters, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Ryan J BartlettDepartment of Chemistry, University at Albany, State University of New York, 1400 Washington Avenue, Albany, New York 12222, United States.

Kelly D CrisostomoDepartment of Chemistry, University at Albany, State University of New York, 1400 Washington Avenue, Albany, New York 12222, United States.

Qiang ZhangDepartment of Chemistry, University at Albany, State University of New York, 1400 Washington Avenue, Albany, New York 12222, United States.ORCID 0000-0003-2787-1781

Funding

The chemical approach towards homogenous glycoprotein preparation and evaluationR35GM138336 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI Qiang Zhang · 2020 to 2026

$2.2M

NIGMS NIH HHS R35 GM138336

6 · The paper itself

Abstract

An investigation of reversible protein conjugation and deconjugation is presented. Despite numerous available protein conjugation methods, there has been limited documentation of achieving protein conjugation in a controlled and reversible manner. This report introduces a protocol that enables protein modification in a multicomponent fashion under aqueous buffer and mild conditions. A readily available mercaptobenzaldehyde derivative can modify the primary amine of peptides and proteins with a distinctive [3.3.1] scaffold. This modification can be reversed under mild conditions in a controlled fashion, restoring the original protein motif. The effectiveness of this approach has been demonstrated in the modification and quantifiable regeneration of insulin protein.

Indexed as

PeptidesProteinsBenzaldehydesInsulinMolecular StructureBenzaldehydesInsulinPeptidesProteins

Identifiers

PMID39038165

DOI10.1021/acs.orglett.4c02228

PMCPMC11301669

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.