ArticleMethods in molecular biology (Clifton, N.J.)2024
Intracardiac Injection Mouse Model to Study Cancer Cell Dormancy in Brain Metastasis.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Protocol for generation of brain metastasis mouse model using stereotactic intracranial tumor-derived cells and modified intracarotid injection.STAR protocols · 2026Article
- Single-cell and spatial transcriptome landscapes reveal the spatial immune defense pattern shared by primary and brain metastatic lung adenocarcinoma.Journal of thoracic disease · 2025Article
- IL6/CCL2 from M2-polarized microglia promotes breast cancer brain metastasis and the reversal effect of β-elemene.Frontiers in pharmacology · 2025Article
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2 authors.
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Abstract
Brain metastasis is a highly complex process, and some cancer cells enter a dormant state after extravasation into the brain. The molecular mechanism of dormancy remains largely unknown and is still under intense investigation. Here, we outline a basic approach to generating and analyzing experimental mouse models to study dormant cancer cells in the brain. Cancer cells stably expressing EGFP and firefly luciferase are injected into the left ventricle of athymic nude mice. After confirmation of brain metastasis by bioluminescence imaging, brain slices are prepared and subjected to Ki67 staining. In addition, a methodology for recovering brain metastatic cancer cells from the mouse brain is described, providing technical tips for unraveling the mysteries of cancer cell dormancy in brain metastasis.
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