Evidence map›Paper›PMID 39037482›Full record

ArticleJournal of microbiology (Seoul, Korea)2024

Whole Genome Sequence Analysis of Brucella spp. from Human, Livestock, and Wildlife in South Africa.

Koketso Desiree Mazwi, Kgaugelo Edward Lekota, Barbara Akofo Glover, Francis Babaman Kolo, Ayesha Hassim, Jenny Rossouw, Annelize Jonker, Justnya Maria Wojno, Giuseppe Profiti, Pier Luigi Martelli and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of microbiology (Seoul, Korea), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. Whole-Genome Sequencing of Brucella melitensis Isolates from Kuwait for the Identification of Biovars, Variants, and Relationship within a Biovar.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Koketso Desiree Mazwi *Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa. desireemazwi@gmail.com.ORCID http://orcid.org/0000-0003-4085-1355
Kgaugelo Edward Lekota *Unit for Environmental Sciences and Management, North-West University, Potchefstroom, 2531, South Africa. lekotaE@gmail.com.
Barbara Akofo GloverDepartment of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa.
Francis Babaman KoloDepartment of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa.
Ayesha HassimDepartment of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa.
Jenny RossouwNational Institute for Communicable Diseases, A Division of the National Health Laboratory Services, Johannesburg, 2192, South Africa.
Annelize JonkerDepartment of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa.
Justnya Maria WojnoLancet Laboratories, Microbiology Laboratory, Century City, Cape Town, 7441, South Africa.
Giuseppe ProfitiBologna Biocomputing Group, University of Bologna, 40126, Bologna, Italy.
Pier Luigi MartelliBologna Biocomputing Group, University of Bologna, 40126, Bologna, Italy.
Rita CasadioBologna Biocomputing Group, University of Bologna, 40126, Bologna, Italy.
Katiuscia ZilliNational and OIE Reference Laboratory for Brucellosis, Experimental Zooprophylactic Institute of Abruzzo and Molise Giuseppe Caporale, 64100, Teramo, Italy.
Anna JanowiczNational and OIE Reference Laboratory for Brucellosis, Experimental Zooprophylactic Institute of Abruzzo and Molise Giuseppe Caporale, 64100, Teramo, Italy.
Francesca MarottaNational and OIE Reference Laboratory for Brucellosis, Experimental Zooprophylactic Institute of Abruzzo and Molise Giuseppe Caporale, 64100, Teramo, Italy.
Giuliano GarofoloNational and OIE Reference Laboratory for Brucellosis, Experimental Zooprophylactic Institute of Abruzzo and Molise Giuseppe Caporale, 64100, Teramo, Italy.
Henriette van HeerdenDepartment of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, 0110, South Africa.

Funding

National Research Foundation 98651
6 · The paper itself

Abstract

Brucellosis is an economically important zoonotic disease affecting humans, livestock, and wildlife health globally and especially in Africa. Brucella abortus and B. melitensis have been isolated from human, livestock (cattle and goat), and wildlife (sable) in South Africa (SA) but with little knowledge of the population genomic structure of this pathogen in SA. As whole genome sequencing can assist to differentiate and trace the origin of outbreaks of Brucella spp. strains, the whole genomes of retrospective isolates (n = 19) from previous studies were sequenced. Sequences were analysed using average nucleotide identity (ANI), pangenomics, and whole genome single nucleotide polymorphism (wgSNP) to trace the geographical origin of cases of brucellosis circulating in human, cattle, goats, and sable from different provinces in SA. Pangenomics analysis of B. melitensis (n = 69) and B. abortus (n = 56) was conducted with 19 strains that included B. abortus from cattle (n = 3) and B. melitensis from a human (n = 1), cattle (n = 1), goat (n = 1), Rev1 vaccine strain (n = 1), and sable (n = 12). Pangenomics analysis of B. melitensis genomes, highlighted shared genes, that include 10 hypothetical proteins and genes that encodes for acetyl-coenzyme A synthetase (acs), and acylamidase (aam) amongst the sable genomes. The wgSNP analysis confirmed the B. melitensis isolated from human was more closely related to the goat from the Western Cape Province from the same outbreak than the B. melitensis cattle sample from different cases in the Gauteng Province. The B. melitensis sable strains could be distinguished from the African lineage, constituting their own African sub-clade. The sequenced B. abortus strains clustered in the C2 lineage that is closely related to the isolates from Mozambique and Zimbabwe. This study identified genetically diverse Brucella spp. among various hosts in SA. This study expands the limited known knowledge regarding the presence of B. melitensis in livestock and humans in SA, further building a foundation for future research on the distribution of the Brucella spp. worldwide and its evolutionary background.

Indexed as

Brucella abortusBrucella melitensisBrucellosisCattleGoatsMustelidaeAbattoirsAnimalsAnimals, WildHumansLivestockMultilocus Sequence TypingPhylogenyPolymorphism, Single NucleotideWhole Genome SequencingB. abortusB. melitensisBrucellosisHumanLivestockWildlife

Identifiers

PMID39037482
PMCPMC11436471

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.