Evidence map›Paper›PMID 39028522›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Unravelling the Biophysical Properties of Chromatin Proteins and DNA Using Acoustic Force Spectroscopy.

Szu-Ning Lin, Liang Qin, Kees-Karel H Taris, Gijs J L Wuite, Remus T Dame

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Szu-Ning LinLeiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Liang QinLeiden Institute of Chemistry, Leiden University, Leiden, The Netherlands.
Kees-Karel H TarisDepartment of Physics and Astronomy, VU University Amsterdam, Amsterdam, The Netherlands.
Gijs J L WuiteDepartment of Physics and Astronomy, VU University Amsterdam, Amsterdam, The Netherlands.
Remus T DameLeiden Institute of Chemistry, Leiden University, Leiden, The Netherlands. rtdame@chem.leidenuniv.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acoustic force spectroscopy (AFS) is a single-molecule micromanipulation technique that uses sound waves to exert force on surface-tethered DNA molecules in a microfluidic chamber. As large numbers of individual protein-DNA complexes are tracked in parallel, AFS provides insight into the individual properties of such complexes as well as their population averages. In this chapter, we describe in detail how to perform AFS experiments specifically on bare DNA, protein-DNA complexes, and how to extract their (effective) persistence length and contour length from force-extension relations.

Indexed as

ChromatinDNAAcousticsDNA-Binding ProteinsMicroscopy, Atomic ForceSingle Molecule ImagingSpectrum AnalysisChromatinDNADNA-Binding ProteinsAcoustic force spectroscopyBacterial chromatin proteinDNA-binding proteinProtein–DNA interactionSingle-molecule manipulation

Identifiers

PMID39028522

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.