Evidence map›Paper›PMID 39028507›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Modular Assembly of Synthetic Secondary Chromosomes.

Celine Zumkeller, Daniel Schindler, Jennifer Felder, Torsten Waldminghaus

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Celine ZumkellerFraunhofer Institute for Molecular Biology and Applied Ecology (IME), Branch for Bioresources, Giessen, Germany.
Daniel SchindlerMax Planck Institute for Terrestrial Microbiology, Marburg, Germany.
Jennifer FelderTechnische Universität Darmstadt, Faculty of Biology, Darmstadt, Germany.
Torsten WaldminghausTechnische Universität Darmstadt, Faculty of Biology, Darmstadt, Germany. torsten.waldminghaus@tu-darmstadt.de.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The development of novel DNA assembly methods in recent years has paved the way for the construction of synthetic replicons to be used for basic research and biotechnological applications. A learning-by-building approach can now answer questions about how chromosomes must be constructed to maintain genetic information. Here we describe an efficient pipeline for the design and assembly of synthetic, secondary chromosomes in Escherichia coli based on the popular modular cloning (MoClo) system.

Indexed as

Escherichia coliSynthetic BiologyChromosomesChromosomes, BacterialCloning, MolecularGenetic EngineeringPlasmidsRepliconDNA librariesEscherichia coliGenome engineeringModular cloningOligo designSynthetic chromosomesSynVicII

Identifiers

PMID39028507

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.