ArticleMicrobiology spectrum2024
A low-cost culture- and DNA extraction-free method for the molecular detection of pneumococcal carriage in saliva.
Article in Microbiology spectrum, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
3 citing papers in PubMed.
- Storage stability of non-encapsulated pneumococci in saliva is dependent on null-capsule clade, with strains carryingMicrobiology (Reading, England) · 2025Article
- An abundance ofMicrobiology (Reading, England) · 2025Article
- Scalable solutions for global health: the SalivaDirect model.Frontiers in cellular and infection microbiology · 2024Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Molecular methods have improved the sensitivity of the detection of pneumococcal carriage in saliva. However, they typically require sample culture enrichment and nucleic acid extraction prior to performing the detection assay and may limit scalability for extensive surveillance of pneumococcus, particularly in low-resource settings. We evaluated the performance of a DNA-extraction-free method for the detection of pneumococcus in saliva. We developed a streamlined qPCR-based protocol for the detection of pneumococcus, omitting culture enrichment and DNA extraction. Using saliva samples collected from children attending childcare centers (New Haven, CT, USA), we evaluated the detection of pneumococcus using saliva lysates as compared to purified DNA extracted from culture-enriched aliquots of the paired samples using qPCR targeting the pneumococcal
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