Evidence map›Paper›PMID 39027666›Full record

ArticleFrontiers in plant science2024

Development of highly discriminatory SCoT- and CBDP-based SCAR fingerprint for authentication of Indian senna (

Sarika Chouksey, Mohd Ashraf Ashfaq, Pushkar Kaira, Sabnam Farhat, Maneesha Pandey, Ch Anil Kumar, Rama Reddy Nagaraja Reddy

Abstract read
In one paragraph

Article in Frontiers in plant science, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

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2citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Sarika ChoukseyBiochemistry Discipline, School of Sciences, Indira Gandhi National Open University, New Delhi, India.
Mohd Ashraf AshfaqDepartment of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India.
Pushkar KairaDepartment of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India.
Sabnam FarhatDepartment of Botany, School of Chemical and Life Sciences, Jamia Hamdard, New Delhi, India.
Maneesha PandeyBiochemistry Discipline, School of Sciences, Indira Gandhi National Open University, New Delhi, India.
Ch Anil KumarBiotechnology, Indian Council of Agricultural Research (ICAR)- Indian Institute of Oilseeds Research, Hyderabad, India.
Rama Reddy Nagaraja ReddyPlant Breeding, Indian Council of Agricultural Research-Directorate of Medicinal and Aromatic Plants Research (ICAR-DMAPR), Boriavi Anand, Gujarat, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: Indian senna ( Methods: In this study, genomic DNA extracted from 44 accessions of Indian senna and four adulterant species was subjected to SCoT and CBDP PCR. The polymorphic amplicons were identified, eluted, ligated, and transformed into Results: Post-sequencing, the sequence of the primary SCoT and CBDP primers was analyzed and extended into the unique signature sequence of the concerned accessions. This resulted in development of one SCoT-44- and two CBDP-25-based SCARs. SCoT-44 SCAR produced a signature amplicon of 287 bp for accession DCA120, and CBDP-25 SCAR yielded signature amplicons of 575 and 345 bp for accessions DCA13 and DCA119, respectively. The developed SCAR markers were validated across 48 samples (44 accessions of Indian senna and 4 adulterant species) and produced distinct amplicons in Indian senna only, while no such amplicon was observed in the other four adulterant species. Discussion: The information generated using these markers have been faithfully converted to single-locus, unequivocal, highly reproducible, and informative sequence-based SCAR markers. These markers will enable discrimination of individual plants on the basis of unique sequence-specific amplicons, which could be used as diagnostic markers to settle issues pertaining to the true identity of Indian senna.

Indexed as

adulterant speciesCBDPIndian sennaSCARSCOTSenna alexandrina Mill.

Identifiers

PMID39027666
PMCPMC11255980

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.