Evidence map›Paper›PMID 39026874›Full record

ArticlebioRxiv : the preprint server for biology2024

Noncanonical roles of ATG5 and membrane atg8ylation in retromer assembly and function.

Masroor Ahmad Paddar, Fulong Wang, Einar S Trosdal, Emily Hendrix, Yi He, Michelle Salemi, Michal Mudd, Jingyue Jia, Thabata L A Duque, Ruheena Javed and 2 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Masroor Ahmad PaddarAutophagy, Inflammation and Metabolism Center of Biochemical Research Excellence.ORCID 0009-0008-5639-3006
Fulong WangAutophagy, Inflammation and Metabolism Center of Biochemical Research Excellence.
Einar S TrosdalAutophagy, Inflammation and Metabolism Center of Biochemical Research Excellence.
Emily HendrixDepartment of Chemistry & Chemical Biology, The University of New Mexico, Albuquerque, NM, USA.
Yi HeDepartment of Chemistry & Chemical Biology, The University of New Mexico, Albuquerque, NM, USA.
Michelle SalemiProteomics Core Facility, UC Davis Genome Center, University of California, Davis, CA 95616, USA.
Michal MuddAutophagy, Inflammation and Metabolism Center of Biochemical Research Excellence.
Jingyue JiaAutophagy, Inflammation and Metabolism Center of Biochemical Research Excellence.
Thabata L A DuqueAutophagy, Inflammation and Metabolism Center of Biochemical Research Excellence.
Ruheena JavedAutophagy, Inflammation and Metabolism Center of Biochemical Research Excellence.
Brett PhinneyProteomics Core Facility, UC Davis Genome Center, University of California, Davis, CA 95616, USA.
Vojo DereticAutophagy, Inflammation and Metabolism Center of Biochemical Research Excellence.ORCID 0000-0002-3624-5208

Funding

Unfolded Protein Response and Autophagy in T Helper Cell Effector FunctionP20GM121176 · NIGMS · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI Samuel Joseph Endicott · 2017 to 2026
$24.9M
Autophagy Against Tuberculosis and HIVR01AI111935 · NIAID · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI DERETIC, VOJO P · 2014 to 2023
$7.0M
Pattern Recognition Receptors and Autophagy in Mtb Control in AIDSR37AI042999 · NIAID · UNIVERSITY OF NEW MEXICO HEALTH SCIS CTR · PI VOJO P DERETIC · 2018 to 2026
$4.7M
Phinney - Orbitrap Fusion ETDS10OD021801 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI PHINNEY, BRETT S · 2016 to 2016
$600k
QE-HF-X Mass SpectrometerS10OD026918 · OD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI PHINNEY, BRETT S · 2020 to 2020
$600k
NIAID NIH HHS R01 AI111935NIAID NIH HHS R37 AI042999NIGMS NIH HHS P20 GM121176NIH HHS S10 OD021801NIH HHS S10 OD026918
6 · The paper itself

Abstract

ATG5 is one of the core autophagy proteins with additional functions such as noncanonical membrane atg8ylation, which among a growing number of biological outputs includes control of tuberculosis in animal models. Here we show that ATG5 associates with retromer's core components VPS26, VPS29 and VPS35 and modulates retromer function. Knockout of ATG5 blocked trafficking of a key glucose transporter sorted by the retromer, GLUT1, to the plasma membrane. Knockouts of other genes essential for membrane atg8ylation, of which ATG5 is a component, affected GLUT1 sorting, indicating that membrane atg8ylation as a process affects retromer function and endosomal sorting. The contribution of membrane atg8ylation to retromer function in GLUT1 sorting was independent of canonical autophagy. These findings expand the scope of membrane atg8ylation to specific sorting processes in the cell dependent on the retromer and its known interactors.

Identifiers

PMID39026874
PMCPMC11257513

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.