Evidence map›Paper›PMID 39026839›Full record

ArticlebioRxiv : the preprint server for biology2024

The nuclear periphery confers repression on H3K9me2-marked genes and transposons to shape cell fate.

Harold Marin, Eric Simental, Charlie Allen, Eric Martin, Barbara Panning, Bassem Al-Sady, Abigail Buchwalter

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Harold MarinCardiovascular Research Institute, University of California, San Francisco, CA, USA.
Eric SimentalDepartment of Microbiology and Immunology, University of California, San Francisco, CA, USA.
Charlie AllenCardiovascular Research Institute, University of California, San Francisco, CA, USA.
Eric MartinCardiovascular Research Institute, University of California, San Francisco, CA, USA.
Barbara PanningDepartment of Biochemistry, University of California, San Francisco, CA, USA.ORCID 0000-0002-8301-1172
Bassem Al-SadyDepartment of Microbiology and Immunology, University of California, San Francisco, CA, USA.ORCID 0000-0002-8996-7941
Abigail BuchwalterCardiovascular Research Institute, University of California, San Francisco, CA, USA.ORCID 0000-0001-7181-6961

Funding

Understanding mechanisms of heterochromatin tethering at the nuclear laminaR35GM142897 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Abigail Lynn Buchwalter · 2021 to 2026
$2.4M
Tracking how molecular machines propagate epigenetic information in time and spaceR35GM141888 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI AL-SADY, BASSEM · 2021 to 2025
$2.3M
OneView 4kX4k sCMOS camera for transmission electron microscopy applicationsS10OD028536 · OD · STANFORD UNIVERSITY · PI MULHOLLAND, JONATHAN W · 2020 to 2020
$195k
NIGMS NIH HHS R35 GM141888NIGMS NIH HHS R35 GM142897NIH HHS S10 OD028536
6 · The paper itself

Abstract

Heterochromatic loci marked by histone H3 lysine 9 dimethylation (H3K9me2) are enriched at the nuclear periphery in metazoans, but the effect of spatial position on heterochromatin function has not been defined. Here, we remove three nuclear lamins and lamin B receptor (LBR) in mouse embryonic stem cells (mESCs) and show that heterochromatin detaches from the nuclear periphery. Mutant mESCs sustain naïve pluripotency and maintain H3K9me2 across the genome but cannot repress H3K9me2-marked genes or transposons. Further, mutant cells fail to differentiate into epiblast-like cells (EpiLCs), a transition that requires the expansion of H3K9me2 across the genome. Mutant EpiLCs can silence naïve pluripotency genes and activate epiblast-stage genes. However, H3K9me2 cannot repress markers of alternative fates, including primitive endoderm. We conclude that the nuclear periphery controls the spatial position, dynamic remodeling, and repressive capacity of H3K9me2-marked heterochromatin to shape cell fate decisions.

Identifiers

PMID39026839
PMCPMC11257442

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.