ArticleDevelopment (Cambridge, England)2024
Stable germline transgenesis using the Minos Tc1/mariner element in the sea urchin Lytechinus pictus.
Article in Development (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed.
- Cryopreservation of sea urchin (Lytechinus pictus) embryos and development through metamorphosis.Developmental dynamics : an official publication of the American Association of Anatomists · 2026Article
- The tuxedo sea urchinbioRxiv : the preprint server for biology · 2026Article
- Efficient Endogenous Tagging in the Sea Urchin,bioRxiv : the preprint server for biology · 2026Article
- Genetically tractable embryonic cell lines from sea urchins Lytechinus variegatus and Strongylocentrotus purpuratus.Communications biology · 2025Article
- Automated, high-throughput in situ hybridization of sea urchin (Lytechinus pictus) embryos.Development (Cambridge, England) · 2025Article
- Article
- Characterization of cellular and molecular immune components of the painted white sea urchin Lytechinus pictus in response to bacterial infection.Immunology and cell biology · 2025Article
- Adapting to change: insights from new organisms in cell and developmental biology.Development (Cambridge, England) · 2024Article
- Localization and origins of juvenile skeletogenic cells in the sea urchin Lytechinuspictus.Developmental biology · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Stable transgenesis is a transformative tool in model organism biology. Although the sea urchin is one of the oldest animal models in cell and developmental biology, studies in this animal have largely relied on transient manipulation of wild animals, without a strategy for stable transgenesis. Here, we build on recent progress to develop a more genetically tractable sea urchin species, Lytechinus pictus, and establish a robust transgene integration method. Three commonly used transposons (Minos, Tol2 and piggyBac) were tested for non-autonomous transposition, using plasmids containing a polyubiquitin promoter upstream of a H2B-mCerulean nuclear marker. Minos was the only transposable element that resulted in significant expression beyond metamorphosis. F0 animals were raised to sexual maturity, and spawned to determine germline integration and transgene inheritance frequency, and to characterize expression patterns of the transgene in F1 progeny. The results demonstrate transgene transmission through the germline, the first example of a germline transgenic sea urchin and, indeed, of any echinoderm. This milestone paves the way for the generation of diverse transgenic resources that will dramatically enhance the utility, reproducibility and efficiency of sea urchin research.
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Registered trials
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