ArticleBiofilm2024
The dynamics of biofilm development and dispersal should be taken into account when quantifying biofilm via the crystal violet microtiter plate assay.
Article in Biofilm, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Beyond lethal doses: sub-MIC levels of multi-class antibiotics induce species- and isolate-dependent biofilm inhibition in gram-negative pathogens.BMC microbiology · 2026Article
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- Probiotic and Antimicrobial Potential of EndophyticMicroorganisms · 2026Article
- The characterization of a new stabilized lactonase from the endophytic fungi Aspergillus pseudonomiae with anti-bacterial and anti-fungal properties.Protein science : a publication of the Protein Society · 2026Article
- Digital Printing of Nanoenabled Enzymes on Textiles: An Integrated Antimicrobial and Antibiofilm Approach.ACS omega · 2026Article
- Surface versus Nanocatalyst-Induced Matrix Bubbles Govern Temperature-Dependent Biofilm Removal.ACS applied materials & interfaces · 2026Article
- Integrated experimental and computational evaluation of Anagallis foemina derived terpenoids against carbapenem resistant Acinetobacter baumannii.Scientific reports · 2026Article
- Measuring Communication in Microbial Biofilms in Response to Antibiotics, Phytochemicals and Stressors.Antioxidants (Basel, Switzerland) · 2026Article
- Unveiling the role of outer membrane proteins (OMPs) in biofilm formation and Harnessing them for targeting biofilm-forming bacterial infections.World journal of microbiology & biotechnology · 2026Review
- Article
- Protocols for Standard and Advanced Visualization and Quantification of Leptospira Biofilms.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Impact of Incremental Antiseptic Selection on Antibiotic Cross-Resistance and Enhanced Biofilm Formation inInternational journal of microbiology · 2026Article
- Impact of hcp and vgrG on Acinetobacter baumannii biofilm formation during infection of human pulmonary alveolar epithelial cells.Scientific reports · 2025Article
- Article
- Antibiofilm Activity ofFoods (Basel, Switzerland) · 2025Article
- Phenotypic and transcriptomic analysis reveals the antibiofilm mechanism ofCurrent research in food science · 2025Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The crystal violet microtiter plate biofilm assay is often used to compare the amount of biofilm formed by a mutant versus wild-type or a compound-treated biofilm versus the non-treatment control. In many of these studies the amount of biofilm is assessed only at one single time point. However, if the dynamics of biofilm development of the mutant (or compound-treated biofilm) is different than that of the wild-type (or non-treatment control), then biofilm quantification at a single time point may give misleading results. To overcome this shortcoming of the common biofilm quantification technique, we recommend to use a serial dilution-based crystal violet microtiter plate biofilm assay for easy assessment of the dynamics of biofilm development and dispersal. We demonstrate that the dilution-resolved crystal violet assay displays the dynamics of
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.