Duplex Real-Time Fluorescent Quantitative PCR Assays for the Detection of Toxigenic Microcystis Genotypes Based on SNP/InDel Variation in mcy Gene Cluster.

Xiangdong Bi, Peng Wei, Qian Wang, Lijin Duan, Wei Dai, Rui Chen, Dajuan Zhang

Abstract read

In one paragraph

Article in Current microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 citing paper in PubMed.

International journal of molecular sciences · 2026
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5 · Who and what money

Authors and funding

7 authors.

Xiangdong BiKey Laboratory of Aquatic-Ecology and Aquaculture of Tianjin, College of Fishery, Tianjin Agricultural University, Tianjin, China.

Peng WeiKey Laboratory of Aquatic-Ecology and Aquaculture of Tianjin, College of Fishery, Tianjin Agricultural University, Tianjin, China.

Qian WangInstitute of Crop Germplasm and Biotechnology, Tianjin Academy of Agricultural Sciences, Tianjin, China.

Lijin DuanInstitute of Crop Germplasm and Biotechnology, Tianjin Academy of Agricultural Sciences, Tianjin, China.

Wei DaiKey Laboratory of Aquatic-Ecology and Aquaculture of Tianjin, College of Fishery, Tianjin Agricultural University, Tianjin, China. daiweitj@126.com.ORCID http://orcid.org/0000-0001-9167-4059

Rui ChenInstitute of Crop Germplasm and Biotechnology, Tianjin Academy of Agricultural Sciences, Tianjin, China.

Dajuan ZhangKey Laboratory of Aquatic-Ecology and Aquaculture of Tianjin, College of Fishery, Tianjin Agricultural University, Tianjin, China.

Funding

National Natural Science Foundation of China No.31772857National Natural Science Foundation of China No.32172978Natural Science Foundation Grant of Tianjin 22JCYBJC00430Science and Technology Department of Gansu Province No.21CX6NP223the Fundamental Research Funds of Tianjin Universities No.2020ZD06the Fundamental Research Funds of Tianjin Universities No.2021KJ110Tianjin Science and Technology Planning Project 22ZYCGSN00050

6 · The paper itself

Abstract

In this study, the toxigenic characteristics of 14 strains of Microcystis were analyzed, and single nucleotide polymorphism (SNP) and insertion/deletion (InDel) loci in microcystin synthetase (mcy) gene clusters were screened. Based on SNP and InDel loci associated with the toxigenic characteristics, primers and TaqMan or Cycling fluorescent probes were designed to develop duplex real-time fluorescent quantitative PCR (FQ-PCR) assays. After evaluating specificity and sensitivity, these assays were applied to detect the toxigenic Microcystis genotypes in a shrimp pond where Microcystis blooms occurred. The results showed a total of 2155 SNP loci and 66 InDel loci were obtained, of which 12 SNP loci and 5 InDel loci were associated with the toxigenic characteristics. Three duplex real-time FQ-PCR assays were developed, each of which could quantify two genotypes of toxigenic Microcystis. These FQ-PCR assays were highly specific, and two Cycling assays were more sensitive than TaqMan assay. In the shrimp pond, six genotypes of toxigenic Microcystis were detected using the developed FQ-PCR assays, indicating that above genotyping assays have the potential for quantitative analysis of the toxigenic Microcystis genotypes in natural water.

Indexed as

GenotypeMicrocystisMultigene FamilyPolymorphism, Single NucleotideReal-Time Polymerase Chain ReactionBacterial ProteinsINDEL MutationMicrocystinsPeptide SynthasesPondsSensitivity and SpecificityBacterial ProteinsMicrocystinsPeptide Synthases

Identifiers

PMID39020143

DOI10.1007/s00284-024-03797-4

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