Evidence map›Paper›PMID 39017982›Full record

ArticlePhotosynthesis research2024

Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium.

Helen W Liu, Eugen I Urzica, Sean D Gallaher, Stefan Schmollinger, Crysten E Blaby-Haas, Masakazu Iwai, Sabeeha S Merchant

Abstract read
PubMed Publisher
In one paragraph

Article in Photosynthesis research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. The Elements of Life, Photosynthesis and Genomics.Journal of molecular biology · 2025
    Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Helen W LiuDepartment of Plant and Microbial Biology, University of California, Berkeley, CA, 99354, USA.
Eugen I UrzicaDepartment of Chemistry and Biochemistry, University of California, Los Angeles, CA, 90095, USA.
Sean D GallaherDepartment of Chemistry and Biochemistry, University of California, Los Angeles, CA, 90095, USA.
Stefan SchmollingerCalifornia Institute for Quantitative Biosciences (QB3), University of California, Berkeley, CA, 94720, USA.
Crysten E Blaby-HaasDepartment of Chemistry and Biochemistry, University of California, Los Angeles, CA, 90095, USA.
Masakazu IwaiMolecular Biophysics and Integrated Bioimaging Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Sabeeha S MerchantDepartment of Plant and Microbial Biology, University of California, Berkeley, CA, 99354, USA. sabeeha@berkeley.edu.

Funding

U.S. Department of Energy DE-SC0020627
6 · The paper itself

Abstract

Low iron (Fe) bioavailability can limit the biosynthesis of Fe-containing proteins, which are especially abundant in photosynthetic organisms, thus negatively affecting global primary productivity. Understanding cellular coping mechanisms under Fe limitation is therefore of great interest. We surveyed the temporal responses of Chlamydomonas (Chlamydomonas reinhardtii) cells transitioning from an Fe-rich to an Fe-free medium to document their short and long-term adjustments. While slower growth, chlorosis and lower photosynthetic parameters are evident only after one or more days in Fe-free medium, the abundance of some transcripts, such as those for genes encoding transporters and enzymes involved in Fe assimilation, change within minutes, before changes in intracellular Fe content are noticeable, suggestive of a sensitive mechanism for sensing Fe. Promoter reporter constructs indicate a transcriptional component to this immediate primary response. With acetate provided as a source of reduced carbon, transcripts encoding respiratory components are maintained relative to transcripts encoding components of photosynthesis and tetrapyrrole biosynthesis, indicating metabolic prioritization of respiration over photosynthesis. In contrast to the loss of chlorophyll, carotenoid content is maintained under Fe limitation despite a decrease in the transcripts for carotenoid biosynthesis genes, indicating carotenoid stability. These changes occur more slowly, only after the intracellular Fe quota responds, indicating a phased response in Chlamydomonas, involving both primary and secondary responses during acclimation to poor Fe nutrition.

Indexed as

Chlamydomonas reinhardtiiIronPhotosynthesisCarotenoidsChlamydomonasChlorophyllGene Expression Regulation, PlantCarotenoidsChlorophyllIronIron and nitrogen comparisonPhotosynthesisSystems biologyTime courseTranscriptional regulation

Identifiers

PMID39017982

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.