Evidence map›Paper›PMID 39017884›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Visualizing Epigenetic Modifications and Nuclear Bodies by Immunofluorescence Staining in Naïve, Activated, and Memory B Cell Subsets.

Clarissa R Chakma, Andrew Garvie, Lee H Wong, Kim L Good-Jacobson

Abstract read
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Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Clarissa R ChakmaDepartment of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia.
Andrew GarvieDepartment of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia.
Lee H WongDepartment of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia. lee.wong@monash.edu.
Kim L Good-JacobsonDepartment of Biochemistry and Molecular Biology, Monash University, Clayton, VIC, Australia. kim.jacobson@monash.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Immunofluorescence microscopy is a powerful technique using fluorescently labelled antibodies which can be used to visualize proteins in the nucleus. A key advantage of this method is that it can provide insight into the spatial organization and the localization of nuclear proteins, which can provide elucidation of their function. Here, we provide a protocol for immunofluorescence staining in the nucleus, which has successfully been used to visualize histone modifications and nuclear bodies in human and mouse B lymphocytes, using as few as 1 × 10

Indexed as

Epigenesis, GeneticFluorescent Antibody TechniqueAnimalsB-Lymphocyte SubsetsCell NucleusHistonesHumansImmunologic MemoryLymphocyte ActivationMiceMicroscopy, FluorescenceStaining and LabelingHistonesConfocal microscopyHistone modificationsImmunofluorescence stainingNuclear bodies

Identifiers

PMID39017884

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.