Evidence map›Paper›PMID 39017814›Full record

ArticleMikrochimica acta2024

Unraveling the influence of CRISPR/Cas13a reaction components on enhancing trans-cleavage activity for ultrasensitive on-chip RNA detection.

Qian He, Qun Chen, Lijin Lian, Jiuxin Qu, Xi Yuan, Chuhui Wang, Lidan Xu, Jiazhang Wei, Shaoling Zeng, Dongmei Yu and 8 more

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Qian HeSchool of Communication Engineering, Hangzhou Dianzi University, Hangzhou, 310018, Zhejiang, China.
Qun ChenInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong Province, China.
Lijin LianInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong Province, China.
Jiuxin QuClinical Laboratory, Shenzhen Third People's Hospital, Shenzhen, 518115, Guangdong Province, China.
Xi YuanInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong Province, China.
Chuhui WangInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong Province, China.
Lidan XuInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong Province, China.
Jiazhang WeiDepartment of Otolaryngology & Head and Neck, The People's Hospital of Guangxi Zhuang Autonomous Region, Guangxi Academy of Medical Sciences, 6 Taoyuan Road, Nanning, 530021, China.
Shaoling ZengAnimal and Plant Inspection and Quarantine Technology Center, Shenzhen Customs, Shenzhen, 518010, Guangdong Province, China.
Dongmei YuSchool of Mechanical, Electrical & Information Engineering, Shandong University, Weihai, 264209, Shandong, China.
Yuhan DongInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong Province, China.
Yongbing ZhangHarbin Institute of Technology (Shenzhen), Shenzhen, 518055, China.
Lin DengInstitute of Molecular Physiology, Shenzhen Bay Laboratory, Shenzhen, 518132, China.
Ke DuChemical and Environmental Engineering, University of California, Riverside, USA.
Canyang ZhangInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong Province, China.
Vijay PandeyInstitute of Biopharmaceutical and Health Engineering, Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen, 518055, Guangdong Province, China.
Ijaz GulSchool of Communication Engineering, Hangzhou Dianzi University, Hangzhou, 310018, Zhejiang, China. gul.ijaz@sz.tsinghua.edu.cn.ORCID http://orcid.org/0000-0002-2929-137X
Peiwu QinSchool of Communication Engineering, Hangzhou Dianzi University, Hangzhou, 310018, Zhejiang, China. pwqin@sz.tsinghua.edu.cn.

Funding

China Postdoctoral Science Foundation 2020M680023Department of Chemical Engineering-iBHE special cooperation joint fund project DCE-iBHE-2022-3Ministry of Land and Resources of the People's Republic of China (2022) 207National Natural Science Foundation of China 31970752National Natural Science Foundation of China 32350410397Science, Technology and Innovation Commission of Shenzhen Municipality JCYJ20190809180003689Science, Technology and Innovation Commission of Shenzhen Municipality JCYJ20230807113017035Science, Technology and Innovation Commission of Shenzhen Municipality JSGG20191129110812708Science, Technology and Innovation Commission of Shenzhen Municipality JSGG20200225150707332; JCYJ20220530143014032Science, Technology and Innovation Commission of Shenzhen Municipality WDZC20200820173710001Shenzhen Bay Laboratory Open Funding SZBL2020090501004Shenzhen Medical Research Funds D2301002Tsinghua Shenzhen International Graduate School JC2022009
6 · The paper itself

Abstract

The CRISPR/Cas13 nucleases have been widely documented for nucleic acid detection. Understanding the intricacies of CRISPR/Cas13's reaction components is pivotal for harnessing its full potential for biosensing applications. Herein, we report on the influence of CRISPR/Cas13a reaction components on its trans-cleavage activity and the development of an on-chip total internal reflection fluorescence microscopy (TIRFM)-powered RNA sensing system. We used SARS-CoV-2 synthetic RNA and pseudovirus as a model system. Our results show that optimizing Mg

Indexed as

CRISPR-Cas SystemsRNA, ViralSARS-CoV-2Biosensing TechniquesCOVID-19COVID-19 Nucleic Acid TestingCRISPR-Associated ProteinsHumansLab-On-A-Chip DevicesLimit of DetectionMagnesiumMicroscopy, FluorescenceCRISPR-Associated ProteinsMagnesiumRNA, ViralAmplification-freeCRISPR/Cas13acrRNA combinationRNA detectionTIRFM

Identifiers

PMID39017814

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.