Evidence map›Paper›PMID 39014212›Full record

ArticleSurgical and radiologic anatomy : SRA2024

Sihler's staining of the anterior belly of digastric muscle for botulinum toxin injection.

You-Jin Choi, Hye-Won Hu, Soo-Bin Kim, Ji-Hyun Lee, Seong-Taek Kim, Hee-Jin Kim

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Article in Surgical and radiologic anatomy : SRA, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

What it found

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

You-Jin ChoiDepartment of Anatomy, School of Medicine, Konkuk University, Chungju, Korea.ORCID http://orcid.org/0000-0003-3701-2200
Hye-Won HuDivision in Anatomy and Developmental Biology, Department of Oral Biology, Human Identification Research Institute, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Korea.ORCID http://orcid.org/0000-0001-5460-6428
Soo-Bin KimDivision in Anatomy and Developmental Biology, Department of Oral Biology, Human Identification Research Institute, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Korea.ORCID http://orcid.org/0000-0003-2576-5723
Ji-Hyun LeeDepartment of Anatomy and Acupoint, College of Korean Medicine, Gachon University, Seongnam, Korea.ORCID http://orcid.org/0000-0003-3732-2698
Seong-Taek KimDepartment of Orofacial Pain & Oral Medicine, Yonsei University College of Dentistry, Seoul, Korea.ORCID http://orcid.org/0000-0001-9506-5103
Hee-Jin KimDivision in Anatomy and Developmental Biology, Department of Oral Biology, Human Identification Research Institute, BK21 FOUR Project, Yonsei University College of Dentistry, Seoul, Korea. hjk776@yuhs.ac.ORCID http://orcid.org/0000-0002-1139-6261

Funding

Korea Medical Device Development Fund 1711138194, KMDF_ PR_ 20200901_0109-01National Research Foundation of Korea RS-2023-00275241
6 · The paper itself

Abstract

purposeThe anterior belly of the digastric muscle (ABDM) is the target of botulinum toxin injection; however, anatomical considerations related to the injection point are absent. This study used Sihler's staining to analyze the intramuscular nerve distribution of ABDM to identify the most effective botulinum toxin injection points.

methodsWe used 12 specimens from 6 embalmed cadavers in this study. The specimens were manually dissected to preserve the mylohyoid nerve and subjected to Sihler's staining. From the gnathion to and hyoid bone, the ABDM was divided into three equal parts, distinguishing the anterior, middle, and posterior thirds.

resultsOnly a branch of the mylohyoid nerve entered the ABDM, and its entry point was located in the middle-third region in all cases. The nerve endings were concentrated in the middle third (100%), followed by the anterior third (58.3%) and were not observed in the posterior third.

conclusionThe landmarks used in this study (gnathion and hyoid bone) are easily palpable on the skin surface, allowing clinicians to target the most effective injection site (middle third of ABDM). These results provide scientific and anatomic evidence for injection points, and will aid in the management of ABDM injection procedures in clinical practice.

Indexed as

CadaverAgedAged, 80 and overAnatomic LandmarksBotulinum ToxinsFemaleHumansInjections, IntramuscularMaleNeck MusclesStaining and LabelingBotulinum ToxinsAnterior belly of digastric muscleBotulinum toxinInjection guidelineSihler’s staining

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.