ArticleRadiology2024
Multidimensional Analysis of the Adult Human Heart in Health and Disease Using Hierarchical Phase-Contrast Tomography.
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15 citing papers in PubMed.
- Evolution of hierarchical phase-contrast tomography on the European Synchrotron beamlines BM05 and BM18: a whole adult human brain imaging case study.Journal of synchrotron radiation · 2026Article
- The heterogeneous nature of atrioventricular conduction tissues in tetralogy of Fallot demonstrated by hierarchical phase-contrast tomography.JTCVS structural and endovascular · 2026Article
- Three-dimensional high-content imaging of unstained soft tissue with subcellular resolution using a laboratory-based X-ray microscope.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- The Human Organ Atlas.Science advances · 2026Article
- The Extremely Brilliant Brain: An Isotropic Microscale Human Brain Dataset.bioRxiv : the preprint server for biology · 2026Article
- Multiscale segmentation using hierarchical phase-contrast tomography and deep learning.PLoS computational biology · 2026Article
- Data-informed reconstruction of a bipennate muscle's aponeurosis and its fibre distribution for performing continuum-mechanical simulations.Biomechanics and modeling in mechanobiology · 2025Article
- The Human Organ Atlas.bioRxiv : the preprint server for biology · 2025Article
- The Anatomy of the Atrioventricular Node.Journal of cardiovascular development and disease · 2025Review
- Article
- High-resolution 3D visualization of human hearts with emphases on the cardiac conduction system components-a new platform for medical education, mix/virtual reality, computational simulation.Frontiers in medicine · 2025Article
- 3D multiscale characterization of the human placenta: Bridging anatomy and histology by X-ray phase-contrast tomography.PNAS nexus · 2025Article
- Relevance of Anatomical Significance of AV Nodal Structures within Koch's Triangle and Pyramid.Journal of cardiovascular development and disease · 2024Review
- Revisiting the Atrioventricular Conduction Axis for the 21st Century.Arrhythmia & electrophysiology review · 2024Review
- The Atrioventricular Conduction Axis Revisited for the 21st Century.Journal of cardiovascular development and disease · 2023Review
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Authors and funding
16 authors.
Joseph BrunetFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0002-8424-9510
Andrew C CookFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0001-5079-7546
Claire L WalshFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0003-3769-3392
James CranleyFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0002-0408-5801
Paul TafforeauFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0002-5962-1683
Klaus EngelFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0009-0001-1423-898X
Owen ArthursFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0003-1213-3516
Camille BerruyerFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).
Emer Burke O'LearyFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0002-8831-8077
Alexandre BellierFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0003-0907-0315
Ryo ToriiFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0001-9479-8719
Christopher WerleinFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0002-7694-4257
Danny D JonigkFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0002-5251-2281
Maximilian AckermannFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).
Kathleen DollmanFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0002-5468-4896
Peter D LeeFrom the Department of Mechanical Engineering, University College London, London, England (J.B., C.L.W., C.B., E.B.O.L., R.T., P.D.L.); European Synchrotron Radiation Facility, 71 Av des Martyrs, 38000 Grenoble, France (J.B., P.T., C.B., K.D.); UCL Institute of Cardiovascular Science, London, England (A.C.C.); Wellcome Sanger Institute, Hinxton, England (J.C.); Siemenst Healthineers, Erlangen, Germany (K.E.); Department of Radiology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, England (O.A.); Laboratoire d'Anatomie des Alpes Françaises, Université Grenoble Alpes, Grenoble, France (A.B.); Institute of Pathology, Hannover Medical School, Hannover, Germany (C.W.); Biomedical Research in Endstage and Obstructive Lung Disease Hannover, German Center for Lung Research (DZL), Hannover, Germany (D.D.J.); Institute of Pathology, Faculty of Medicine, RWTH Aachen University, Aachen, Germany (D.D.J., M.A.); Institute of Pathology and Molecular Pathology, Helios University Clinic Wuppertal, Universität Witten/Herdecke, Wuppertal, Germany (M.A.); Institute of Functional and Clinical Anatomy, University Medical Center of the Johannes Gutenberg-University Mainz, Mainz, Germany (M.A.); and Research Complex at Harwell, Didcot, England (P.D.L.).ORCID 0000-0002-3898-8881
Funding
Adult Tissue Morphogenesis: Functional Regulation of Intussusceptive AngiogenesisR01HL094567 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI MENTZER, STEVEN · 2009 to 2016
$3.2MFormation of the Elastin Line Element in the Developing LungF32HL134229 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI VALENZUELA, CRISTIAN DAVID · 2016 to 2016
$57kNHLBI NIH HHS F32 HL134229NHLBI NIH HHS R01 HL094567Wellcome Trust
Abstract
Background Current clinical imaging modalities such as CT and MRI provide resolution adequate to diagnose cardiovascular diseases but cannot depict detailed structural features in the heart across length scales. Hierarchical phase-contrast tomography (HiP-CT) uses fourth-generation synchrotron sources with improved x-ray brilliance and high energies to provide micron-resolution imaging of intact adult organs with unprecedented detail. Purpose To evaluate the capability of HiP-CT to depict the macro- to microanatomy of structurally normal and abnormal adult human hearts ex vivo. Materials and Methods Between February 2021 and September 2023, two adult human donor hearts were obtained, fixed in formalin, and prepared using a mixture of crushed agar in a 70% ethanol solution. One heart was from a 63-year-old White male without known cardiac disease, and the other was from an 87-year-old White female with a history of multiple known cardiovascular pathologies including ischemic heart disease, hypertension, and atrial fibrillation. Nondestructive ex vivo imaging of these hearts without exogenous contrast agent was performed using HiP-CT at the European Synchrotron Radiation Facility. Results HiP-CT demonstrated the capacity for high-spatial-resolution, multiscale cardiac imaging ex vivo, revealing histologic-level detail of the myocardium, valves, coronary arteries, and cardiac conduction system across length scales. Virtual sectioning of the cardiac conduction system provided information on fatty infiltration, vascular supply, and pathways between the cardiac nodes and adjacent structures. HiP-CT achieved resolutions ranging from gross (isotropic voxels of approximately 20 µm) to microscopic (approximately 6.4-µm voxel size) to cellular (approximately 2.3-µm voxel size) in scale. The potential for quantitative assessment of features in health and disease was demonstrated. Conclusion HiP-CT provided high-spatial-resolution, three-dimensional images of structurally normal and diseased ex vivo adult human hearts. Whole-heart image volumes were obtained with isotropic voxels of approximately 20 µm, and local regions of interest were obtained with resolution down to 2.3-6.4 µm without the need for sectioning, destructive techniques, or exogenous contrast agents. Published under a CC BY 4.0 license
Indexed as
HeartTomography, X-Ray ComputedAged, 80 and overFemaleHeart DiseasesHumansMaleMiddle AgedSynchrotrons
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