ArticleFrontiers in cellular and infection microbiology2024
Application of metagenomic next-generation sequencing for rapid molecular identification in spinal infection diagnosis.
Article in Frontiers in cellular and infection microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Comparative diagnostic performance of metagenomic next-generation sequencing and conventional microbial culture in spinal infections: a systematic review and meta-analysis.Frontiers in cellular and infection microbiology · 2026Pooled it
- Recommendations of the International Consensus Meeting (ICM) 2025 on Spinal Infections: Part A Pyogenic Spondylodiscitis.Global spine journal · 2026Article
- [A clinical auxiliary differential diagnostic model for thoracolumbar spinal tuberculosis based on routine laboratory indicators: A multicenter retrospective cohort study].Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2026Article
- Comparative analysis of pathogen detection methods in spinal infections: a retrospective study of 287 cases.BMC infectious diseases · 2026Article
- Metagenomic and Targeted Next-Generation Sequencing in Infectious Disease Diagnostics: Current Applications, Challenges, and Future Perspectives.Diagnostics (Basel, Switzerland) · 2026Review
- C-arm-guided percutaneous biopsy combined with mNGS: a dual-modality strategy for optimizing diagnosis and targeted management of spinal infections.European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society · 2026Review
- Efficacy and safety of an all-oral delamanid-containing regimen in the treatment of multidrug-resistant pulmonary tuberculosis complicated by extrapulmonary tuberculosis: Four case reports and review of the literature.Journal of clinical tuberculosis and other mycobacterial diseases · 2026Article
- A four-year misdiagnosis of spinalIDCases · 2026Article
- Integrating metagenomic next-generation sequencing into a multimodal diagnostic framework for spinal infection: enhancing etiological identification and clinical prediction.Frontiers in cellular and infection microbiology · 2026Article
- Clinical Characteristics and Risk Factors Analysis of Nontuberculous Mycobacterial Pulmonary Disease Complicated with Bronchiectasis.Infection and drug resistance · 2026Article
- Comparative analysis of microbial diversity and clinical outcomes in critically ill patients with and without malignancies: a single-center retrospective cohort study.Frontiers in microbiology · 2026Article
- Review
- Combined posterior-anterior surgery with autologous bone grafting for multi-segmental spinal tuberculosis.European journal of medical research · 2025Article
- Uniportal endoscopic decompression and debridement for infectious diseases of spine with neurological deficits: a retrospective study in China.Asian spine journal · 2025Article
- Diagnostic utility of metagenomic next-generation sequencing for tissue in patients with suspected infectious diseases.Frontiers in cellular and infection microbiology · 2025Article
- Clinical application of metagenomic next-generation sequencing in the diagnosis of severe pneumonia pathogens.Frontiers in cellular and infection microbiology · 2025Article
- Research progress of metagenomic next-generation sequencing in infectious diseases of the spine: a systematic review.Therapeutic advances in infectious diseaseReview
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objective: This study aimed to determine the sensitivity and specificity of metagenomic next-generation sequencing (mNGS) for detecting pathogens in spinal infections and to identify the differences in the diagnostic performance between mNGS and targeted next-generation sequencing (tNGS). Methods: A total of 76 consecutive patients with suspected spinal infections who underwent mNGS, culture, and histopathological examinations were retrospectively studied. The final diagnosis of the patient was determined by combining the clinical treatment results, pathological examinations, imaging changes and laboratory indicators. The sensitivity and specificity of mNGS and culture were determined. Results: The difference between the two detection rates was statistically significant ( Conclusion: mNGS for spinal infection demonstrated better diagnostic value in developing an antibiotic regimen earlier, and it is recommended to prioritize pus samples for testing through mNGS. Moreover, tNGS outperformed other methods for diagnosing spinal TB and identifying antibiotic-resistance genes in drug-resistant TB.
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