Evidence map›Paper›PMID 39010882›Full record

ArticleJournal of structural biology: X2024

Magic-angle spinning NMR structure of Opa60 in lipid bilayers.

Marcel C Forster, Kumar Tekwani Movellan, Eszter E Najbauer, Stefan Becker, Loren B Andreas

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of structural biology: X, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Marcel C ForsterDepartment of NMR-based Structural Biology, Max-Planck-Institute for Multidisciplinary Sciences, Am Faßberg 11, 37077 Göttingen, Germany.
Kumar Tekwani MovellanDepartment of NMR-based Structural Biology, Max-Planck-Institute for Multidisciplinary Sciences, Am Faßberg 11, 37077 Göttingen, Germany.
Eszter E NajbauerDepartment of NMR-based Structural Biology, Max-Planck-Institute for Multidisciplinary Sciences, Am Faßberg 11, 37077 Göttingen, Germany.
Stefan BeckerDepartment of NMR-based Structural Biology, Max-Planck-Institute for Multidisciplinary Sciences, Am Faßberg 11, 37077 Göttingen, Germany.
Loren B AndreasDepartment of NMR-based Structural Biology, Max-Planck-Institute for Multidisciplinary Sciences, Am Faßberg 11, 37077 Göttingen, Germany.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here we report the structure of Opa60 in lipid bilayers using proton-detected magic-angle spinning nuclear magnetic resonance (MAS NMR). Preparations including near-native oligosaccharide lipids reveal a consistent picture of a stable transmembrane beta barrel with a minor increase in the structured region as compared with the previously reported detergent structure. The large variable loops known to interact with host proteins could not be detected, confirming their dynamic nature even in a lipid bilayer environment. The structure provides a starting point for investigation of the functional role of Opa60 in gonococcal infection, which is understood to involve interaction with host proteins. At the same time, it demonstrates the recent advances in proton-detected methodology for membrane protein structure determination at atomic resolution by MAS NMR.

Indexed as

Atomic resolution structureMagic-angle spinningMembrane proteinOpacity associated proteinSolid-state NMR

Identifiers

PMID39010882
PMCPMC11247266

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.