ArticleNature communications2024
Discovery of a terpene synthase synthesizing a nearly non-flexible eunicellane reveals the basis of flexibility.
Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Reprogramming product selectivity and activity in promiscuous terpene synthases via substrate conformation engineering.Nature communications · 2026Article
- Functional Characterization and Mutagenesis Studies of a Microbial-like Diterpene Synthase fromMolecules (Basel, Switzerland) · 2026Article
- Biosynthetic formation of the 6/7/5-odyverdiene diterpenes via a 6/10-ACS catalysis · 2026Article
- A Single Residue Switch Controls Isomer Selectivity in the Myxobacterial Isobonnadiene Synthase.Organic letters · 2026Article
- Mechanistic Characterisation of Bacterial Terpene Synthases from Chitinophagaceae Producing Marine-Type Diterpenes.Angewandte Chemie (International ed. in English) · 2025Article
- Vinigrol Tricyclic Scaffold Biosynthesis Employs an Atypical Terpene Cyclase and a Multipotent Cyclization Cascade.Journal of the American Chemical Society · 2025Article
- Crystal Structure and Catalytic Mechanism of the cis-Eunicellane Cyclase AriE.Chemistry (Weinheim an der Bergstrasse, Germany) · 2025Article
- Dual distal insertions drive pimarane-to-miltiradiene rearrangement with enhanced enzymatic activity.Biodesign research · 2025Article
- Antibacterial Methyl Ester Cembranoids from the Soft CoralMarine drugs · 2025Article
- Mining coral-derived terpene synthases and mechanistic studies of the coral biflorane synthase.Science advances · 2025Article
- Discovery of MK8383s with Antifungal Activity from Mangrove Endophytic FungiMarine drugs · 2025Article
- Discovery of bacterial terpenoids by genome mining.Methods in enzymology · 2025Article
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11 authors.
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Abstract
Eunicellane diterpenoids, containing a typical 6,10-bicycle, are bioactive compounds widely present in marine corals, but rarely found in bacteria and plants. The intrinsic macrocycle exhibits innate structural flexibility resulting in dynamic conformational changes. However, the mechanisms controlling flexibility remain unknown. The discovery of a terpene synthase, MicA, that is responsible for the biosynthesis of a nearly non-flexible eunicellane skeleton, enable us to propose a feasible theory about the flexibility in eunicellane structures. Parallel studies of all eunicellane synthases in nature discovered to date, including 2Z-geranylgeranyl diphosphate incubations and density functional theory-based Boltzmann population computations, reveale that a trans-fused bicycle with a 2Z-configuration alkene restricts conformational flexibility resulting in a nearly non-flexible eunicellane skeleton. The catalytic route and the enzymatic mechanism of MicA are also elucidated by labeling experiments, density functional theory calculations, structural analysis of the artificial intelligence-based MicA model, and mutational studies.
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