Evidence map›Paper›PMID 39009379›Full record

ArticleRNA (New York, N.Y.)2024

Kinetic dissection of pre-crRNA binding and processing by CRISPR-Cas12a.

Selma Sinan, Nathan M Appleby, Chia-Wei Chou, Ilya J Finkelstein, Rick Russell

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA (New York, N.Y.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Molecular mechanisms and biotechnology applications of CRISPR-Cas12a.Nature reviews. Molecular cell biology · 2026
    Review
  2. Article
  3. Article
  4. Review
  5. Article
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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Selma SinanDepartment of Molecular Biosciences, University of Texas at Austin, Austin, Texas 78712, USA.ORCID 0000-0002-0632-3658
Nathan M ApplebyDepartment of Molecular Biosciences, University of Texas at Austin, Austin, Texas 78712, USA.ORCID 0009-0005-5634-7366
Chia-Wei ChouDepartment of Molecular Biosciences, University of Texas at Austin, Austin, Texas 78712, USA.ORCID 0000-0001-9618-338X
Ilya J FinkelsteinDepartment of Molecular Biosciences, University of Texas at Austin, Austin, Texas 78712, USA.
Rick RussellDepartment of Molecular Biosciences, University of Texas at Austin, Austin, Texas 78712, USA rick_russell@cm.utexas.edu.ORCID 0000-0002-4879-8563

Funding

Mechanisms and Specificity of Proteins that Manipulate DNA and RNA StructureR35GM131777 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI Rick Russell · 2019 to 2026
$3.2M
Mechanism, specificity, and design of CRISPR RNA-mediated gene regulationR01GM124141 · NIGMS · UNIVERSITY OF TEXAS AT AUSTIN · PI FINKELSTEIN, ILYA J · 2017 to 2020
$1.2M
NIGMS NIH HHS R01 GM124141NIGMS NIH HHS R35 GM131777
6 · The paper itself

Abstract

CRISPR-Cas12a binds and processes a single pre-crRNA during maturation, providing a simple tool for genome editing applications. Here, we constructed a kinetic and thermodynamic framework for pre-crRNA processing by Cas12a in vitro, and we measured the contributions of distinct regions of the pre-crRNA to this reaction. We find that the pre-crRNA binds rapidly and extraordinarily tightly to Cas12a (

Indexed as

CRISPR-Cas SystemsRNA, Guide, CRISPR-Cas SystemsBacterial ProteinsBase SequenceCRISPR-Associated ProteinsEndodeoxyribonucleasesGene EditingKineticsNucleic Acid ConformationProtein BindingThermodynamicsBacterial ProteinsCas12a proteinCRISPR-Associated ProteinsEndodeoxyribonucleasesRNA, Guide, CRISPR-Cas SystemsAsCas12aCRISPR–Cas systemrate-limiting bindingRNA foldingRNA-guided nuclease

Identifiers

PMID39009379
PMCPMC11404446

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.