Evidence map›Paper›PMID 39008254›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Recombinant Biogenesis and Analysis of Cytochrome c Species.

Alicia N Kreiman, Tania Yeasmin, Molly C Sutherland

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. TheCurrent research in microbial sciences · 2025
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Alicia N KreimanDepartment of Biological Sciences, University of Delaware, Newark, DE, USA.ORCID 0000-0001-6778-3335
Tania YeasminDepartment of Chemistry and Biochemistry, University of Delaware, Newark, DE, USA.ORCID 0000-0003-3039-1818
Molly C SutherlandDepartment of Biological Sciences, University of Delaware, Newark, DE, USA. msuther@udel.edu.ORCID 0000-0002-7932-5339

Funding

Chemistry-Biology Interface Predoctoral Training Grant 2024-2029T32GM133395 · NIGMS · UNIVERSITY OF DELAWARE · PI Catherine Leimkuhler Grimes · 2019 to 2026
$3.7M
Heme trafficking in prokaryotic cytochrome c biogenesisR35GM142496 · NIGMS · UNIVERSITY OF DELAWARE · PI SUTHERLAND, MOLLY CUDDY · 2021 to 2025
$2.0M
NIGMS NIH HHS R35 GM142496NIGMS NIH HHS T32 GM133395
6 · The paper itself

Abstract

Recombinant expression and biogenesis of cytochrome c species is a simple and efficient method for the production of holocytochrome c species, thus presenting an avenue for the study of cytochrome c or the cytochrome c biogenesis pathways responsible for heme attachment. Here, we describe a method for recombinant E. coli production of holocytochrome c utilizing the System I (CcmABCDEFGH) bacterial cytochrome c biogenesis pathway, followed by analysis of cytochrome c species by cell lysis and heme stain.

Indexed as

Cytochromes cEscherichia coliHemeRecombinant ProteinsCytochromes cHemeRecombinant ProteinsCytochrome cCytochrome c biogenesisHemeHeme stain

Identifiers

PMID39008254

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.