Evidence map›Paper›PMID 39008253›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Fluorometric Methods to Measure Bioavailable and Total Heme.

Iramofu M Dominic, Mathilda M Willoughby, Abigail K Freer, Courtney M Moore, Rebecca K Donegan, Osiris Martinez-Guzman, David A Hanna, Amit R Reddi

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Iramofu M DominicSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Mathilda M WilloughbySchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Abigail K FreerSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Courtney M MooreSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Rebecca K DoneganDepartment of Chemistry, Barnard College, New York, NY, USA.
Osiris Martinez-GuzmanSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
David A HannaSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA.
Amit R ReddiSchool of Chemistry and Biochemistry, Georgia Institute of Technology, Atlanta, GA, USA. amit.reddi@chemistry.gatech.edu.

Funding

Defective heme transport in the development of congenital hydrocephalusR01NS123168 · NINDS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ARNOLD, THOMAS DARMODY · 2021 to 2025
$3.1M
Illuminating Heme Trafficking and Signaling Pathways in Health and DiseaseR35GM145350 · NIGMS · GEORGIA INSTITUTE OF TECHNOLOGY · PI Amit Ram Reddi · 2022 to 2026
$1.9M
Imaging heme based mitochondrial-cell signaling networks in cell and animal modelR33ES025661 · NIEHS · GEORGIA INSTITUTE OF TECHNOLOGY · PI REDDI, AMIT RAM · 2017 to 2019
$1.7M
Imaging heme based mitochondrial-cell signaling networks in cell and animal models of heavy metal toxicityR21ES025661 · NIEHS · GEORGIA INSTITUTE OF TECHNOLOGY · PI REDDI, AMIT RAM · 2015 to 2016
$416k
NIEHS NIH HHS R21 ES025661NIEHS NIH HHS R33 ES025661NIGMS NIH HHS R35 GM145350NINDS NIH HHS R01 NS123168US National Science Foundation 1552791
6 · The paper itself

Abstract

Heme b (iron protoporphyrin IX) is an essential but potentially cytotoxic cofactor, signaling molecule, and nutritional source of iron. Its importance in cell biology and metabolism is underscored by the fact that numerous diseases, including various cancers, neurodegenerative disorders, infectious diseases, anemias, and porphyrias, are associated with the dysregulation of heme synthesis, degradation, trafficking, and/or transport. Consequently, methods to measure, image, and quantify heme in cells are required to better understand the physiology and pathophysiology of heme. Herein, we describe fluorescence-based protocols to probe heme bioavailability and trafficking dynamics using genetically encoded fluorescent heme sensors in combination with various modalities, such as confocal microscopy, flow cytometry, and microplate readers. Additionally, we describe a protocol for measuring total heme and its precursor protoporphyrin IX using a fluorometric assay that exploits porphyrin fluorescence. Together, the methods described enable the monitoring of total and bioavailable heme to study heme homeostatic mechanisms in virtually any cell type and organism.

Indexed as

FluorometryHemeAnimalsBiological AvailabilityFlow CytometryHumansMicroscopy, ConfocalProtoporphyrinsHemeprotoporphyrin IXProtoporphyrinsBioavailable hemeFluorescent heme sensorsHemeIronLabile hemePorphyrin

Identifiers

PMID39008253

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.