ArticleMethods in molecular biology (Clifton, N.J.)2024
Sample Preparation for X-Ray Fluorescence Microscopy of Iron Distribution in Biological Specimen.
Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- ZFT is the major iron and zinc transporter ineLife · 2026Article
- Tools to study microbial iron homeostasis and oxidative stress: current techniques and methodological gaps.Frontiers in molecular biosciences · 2025Review
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Authors and funding
2 authors.
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Abstract
Characterizing the two- and three-dimensional distribution of trace metals in biological specimens is key to better understand their role in biological processes. Iron (Fe) is of particular interest in these trace metals due to its widespread role in maintaining cellular health and preventing disease. X-ray fluorescence microscopy (XFM) is emerging as the method of choice for investigators to interrogate the cellular and subcellular distribution of Fe. XFM utilizes the intrinsic X-ray fluorescence properties of each element to produce quantitative 2D and 3D distributions of trace metals within a sample. Herein, methods for sample preparation of cells and tissue for the determination of Fe distribution by XFM are described.
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