Mechanical Compression of Drosophila Embryos Using Rapid Fabrication Microfluidic Devices.

Megan Levis, Fabio Sacco, Vijay Velagala, Fernando Ontiveros, Jeremiah J Zartman

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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Megan LevisDepartment of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN, USA.

Fabio SaccoBiology Department, St. John Fisher University , Rochester, NY, USA.

Vijay VelagalaDepartment of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN, USA.

Fernando OntiverosBiology Department, St. John Fisher University , Rochester, NY, USA.

Jeremiah J ZartmanDepartment of Chemical and Biomolecular Engineering, University of Notre Dame, Notre Dame, IN, USA. jzartman@nd.edu.

Funding

Regulation and function of multicellular calcium signaling in epithelial growth and regenerationR35GM124935 · NIGMS · UNIVERSITY OF NOTRE DAME · PI ZARTMAN, JEREMIAH JAMES · 2017 to 2021

$1.9M

National Science Foundation CBET-1553826NIGMS NIH HHS R35 GM124935

6 · The paper itself

Abstract

Microfluidic devices support developmental and mechanobiology studies by enabling the precise control of electrical, chemical, and mechanical stimuli at the microscale. Here, we describe the fabrication of customizable microfluidic devices and demonstrate their efficacy in applying mechanical loads to micro-organs and whole organisms, such as Drosophila embryos. The fabrication technique consists in the use of xurography to define channels and chambers using thin layers of thermoplastics and glass. The superposition of layers followed by thermal lamination produces robust and reproducible devices that are easily adapted for a variety of experiments. The integration of deformable layers and glass in these devices facilitates the imaging of cellular and molecular dynamics in biological specimens under mechanical loads. The method is highly adaptable for studies in mechanobiology.

Indexed as

Embryo, NonmammalianLab-On-A-Chip DevicesAnimalsBiomechanical PhenomenaDrosophilaDrosophila melanogasterEquipment DesignMicrofluidic Analytical TechniquesStress, MechanicalMechanobiologyModel organismsMorphogenesisOrgan development

Identifiers

PMID39008180

DOI10.1039/C8LC01055B

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.