Evidence map›Paper›PMID 39008177›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Bioprinting Cell-Laden Hydrogels for Studies of Epithelial Tissue Morphogenesis.

Bryan A Nerger, Celeste M Nelson

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Bryan A NergerDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ, USA.
Celeste M NelsonDepartment of Chemical and Biological Engineering, Princeton University, Princeton, NJ, USA. celesten@princeton.edu.

Funding

Biophysical and Biochemical Effects of the ECM on Breast Epithelial CellsR01CA187692 · NCI · PRINCETON UNIVERSITY · PI NELSON, CELESTE M, RADISKY, DEREK C · 2015 to 2019
$2.1M
Mechanical Regulation of Mesenchyme and Mammalian Lung DevelopmentR01HL120142 · NHLBI · PRINCETON UNIVERSITY · PI NELSON, CELESTE M · 2014 to 2017
$1.6M
Exogenous Fluid Forces and Branching of the Mammalian LungR21HL118532 · NHLBI · PRINCETON UNIVERSITY · PI NELSON, CELESTE M · 2014 to 2015
$442k
NCI NIH HHS R01 CA187692NHLBI NIH HHS R01 HL120142NHLBI NIH HHS R21 HL118532
6 · The paper itself

Abstract

The extracellular matrix (ECM) provides dynamic structural and molecular signals that affect the form and function of developing tissues. In order to parse how the individual features of the ECM impact cell- and tissue-level behavior during development, engineered culture models should reproduce key structural and molecular features of native ECM. Here, we describe a protocol for bioprinting epithelial cell aggregates embedded within a collagen-Matrigel ink in order to study the dynamic interplay between epithelial tissues and aligned networks of type I collagen fibers. Collagen fiber alignment and geometry can be spatially controlled by modulating the printing speed, nozzle geometry, surface chemistry, and degree of molecular crowding in the printing ink. We provide detailed procedures for generating epithelial cell aggregates, microextrusion printing collagen-Matrigel bioinks, culturing the three-dimensional (3D)-printed tissues, and imaging 3D-printed collagen-Matrigel constructs.

Indexed as

BioprintingCollagenEpithelial CellsExtracellular MatrixHydrogelsPrinting, Three-DimensionalTissue EngineeringAnimalsDogsDrug CombinationsEpitheliumHumansLamininMorphogenesisProteoglycansTissue ScaffoldsCollagenDrug CombinationsHydrogelsLamininmatrigelProteoglycans3D printingBiomaterialFlow-induced alignment

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.