Evidence map›Paper›PMID 39008174›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Scalable Generation of 3D Pancreatic Islet Organoids from Human Pluripotent Stem Cells in Suspension Bioreactors.

Samuel D Pollock, Israeli M Galicia-Silva, Mai Liu, Zoe L Gruskin, Juan R Alvarez-Dominguez

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. The Last Mile in Beta-Cell Replacement Therapy for Type 1 Diabetes: Time to Grow Up.Transplant international : official journal of the European Society for Organ Transplantation · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Samuel D PollockDepartment of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Israeli M Galicia-SilvaDepartment of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Mai LiuInstitute for Regenerative Medicine and Institute for Diabetes, Obesity and Metabolism, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Zoe L GruskinDepartment of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Juan R Alvarez-DominguezDepartment of Cell and Developmental Biology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA. juan.alvaraz@pennmedicine.upenn.edu.

Funding

Renewal of the Human Islet Research Enhancement Center (HIREC) for the Type-1-Diabetes-Focused Human Islet Research Network (HIRN).U24DK104162 · NIDDK · BECKMAN RESEARCH INSTITUTE/CITY OF HOPE · PI John S. Kaddis, Joyce Carol Niland · 2019 to 2026
$12.3M
Charting human islet maturation via combined soft nanoelectronics and single-cell spatial transcriptomicsDP1DK130673 · NIDDK · HARVARD UNIVERSITY · PI LIU, JIA · 2021 to 2025
$4.3M
Circadian Control of Pancreatic Beta-cell Maturation by Dec1K01DK129442 · NIDDK · UNIVERSITY OF PENNSYLVANIA · PI ALVAREZ, JUAN R · 2021 to 2023
$580k
NIDDK NIH HHS DP1 DK130673NIDDK NIH HHS K01 DK129442NIDDK NIH HHS U24 DK104162
6 · The paper itself

Abstract

We describe a scalable method for the robust generation of 3D pancreatic islet-like organoids from human pluripotent stem cells using suspension bioreactors. Our protocol involves a 6-stage, 20-day directed differentiation process, resulting in the production of 10

Indexed as

BioreactorsCell Culture TechniquesCell DifferentiationIslets of LangerhansOrganoidsPluripotent Stem CellsCryopreservationHumans3D Tissue engineeringBioreactorsDirected stem cell differentiationGlucose-stimulated hormone secretionOrganoidsPancreatic islet beta cells

Identifiers

PMID39008174

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.