Evidence map›Paper›PMID 39008123›Full record

ArticleMicrobial ecology2024

Metagenomic Analysis of Rhizospheric Bacterial Community of Citrus Trees Expressing Phloem-Directed Antimicrobials.

Leandro Alberto Núñez-Muñoz, Martín Eduardo Sánchez-García, Berenice Calderón-Pérez, Rodolfo De la Torre-Almaraz, Roberto Ruiz-Medrano, Beatriz Xoconostle-Cázares

Abstract read
In one paragraph

Article in Microbial ecology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Leandro Alberto Núñez-MuñozDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, 07360, Mexico City, Mexico.ORCID http://orcid.org/0000-0002-5680-1945
Martín Eduardo Sánchez-GarcíaDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, 07360, Mexico City, Mexico.ORCID http://orcid.org/0009-0004-4544-055X
Berenice Calderón-PérezDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, 07360, Mexico City, Mexico.ORCID http://orcid.org/0000-0002-6144-1411
Rodolfo De la Torre-AlmarazFacultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, 54090, Mexico City, Estado de México, Mexico.ORCID http://orcid.org/0000-0001-5763-1231
Roberto Ruiz-MedranoDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, 07360, Mexico City, Mexico.ORCID http://orcid.org/0000-0003-0223-7776
Beatriz Xoconostle-CázaresDepartamento de Biotecnología y Bioingeniería, Centro de Investigación y de Estudios Avanzados, Av. Instituto Politécnico Nacional 2508, Col. San Pedro Zacatenco, 07360, Mexico City, Mexico. bxoconos@cinvestav.mx.ORCID http://orcid.org/0000-0002-7366-7725

Funding

Consejo Nacional de Humanidades, Ciencias y Tecnologías CF-2023-G-231Servicio Nacional de Sanidad e Inocuidad Agroalimentaria (SENASICA) SENASICA-CINVESTAV 2017
6 · The paper itself

Abstract

Huanglongbing, also known as citrus greening, is currently the most devastating citrus disease with limited success in prevention and mitigation. A promising strategy for Huanglongbing control is the use of antimicrobials fused to a carrier protein (phloem protein of 16 kDa or PP16) that targets vascular tissues. This study investigated the effects of genetically modified citrus trees expressing Citrus sinensis PP16 (CsPP16) fused to human lysozyme and β-defensin-2 on the soil microbiome diversity using 16S amplicon analysis. The results indicated that there were no significant alterations in alpha diversity, beta diversity, phylogenetic diversity, differential abundance, or functional prediction between the antimicrobial phloem-overexpressing plants and the control group, suggesting minimal impact on microbial community structure. However, microbiota diversity analysis revealed distinct bacterial assemblages between the rhizosphere soil and root environments. This study helps to understand the ecological implications of crops expressing phloem-targeted antimicrobials for vascular disease management, with minimal impact on soil microbiota.

Indexed as

BacteriaCitrusMicrobiotaPhloemPlant DiseasesRhizosphereSoil MicrobiologyAnti-Infective Agentsbeta-DefensinsCitrus sinensisMetagenomicsMuramidasePhylogenyPlant ProteinsPlant RootsPlants, Genetically ModifiedAnti-Infective Agentsbeta-DefensinsMuramidasePlant ProteinsRNA, Ribosomal, 16SHLB mitigationMicrobial diversityPhloem-directed antimicrobialsTransgenic citrus

Identifiers

PMID39008123
PMCPMC11249458

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.