Evidence map›Paper›PMID 39007315›Full record

ArticleWater science and technology : a journal of the International Association on Water Pollution Research2024

Evaluating storage conditions and enhancement strategies on viral biomarker recovery for WBE applications.

Sueyanka Subroyen, Leanne Pillay, Faizal Bux, Sheena Kumari

Abstract read
PubMed Publisher
In one paragraph

Article in Water science and technology : a journal of the International Association on Water Pollution Research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sueyanka SubroyenInstitute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa.ORCID https://orcid.org/0000-0001-8707-4499
Leanne PillayInstitute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa.ORCID https://orcid.org/0000-0002-7257-5095
Faizal BuxInstitute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa.ORCID https://orcid.org/0000-0002-8108-0238
Sheena KumariInstitute for Water and Wastewater Technology, Durban University of Technology, P.O. Box 1334, Durban 4000, South Africa E-mail: sheenak1@dut.ac.za.ORCID https://orcid.org/0000-0002-3436-8263

Funding

National Research Foundation of South Africa competitive funding UID: 129358South African Research Chair Initiative UID: 84166
6 · The paper itself

Abstract

Wastewater-based epidemiology (WBE) is a valuable disease surveillance tool. However, little is known on how factors such as transportation, storage, and wastewater characteristics influence the accuracy of the quantification methods. Hence, this study investigated the impact of storage temperatures and physicochemical characteristics of wastewater on SARS-CoV-2 and influenza A stability using droplet digital PCR. Additionally, strategies to enhance viral recovery were explored. Municipal influent wastewater stored between ±25 and -80 °C was assessed for a period of 84 days to determine viral degradation. Degradation up to 94.1% of influenza A and SARS-CoV-2 was observed in all samples with the highest at ±25 °C. Viral degradation was correlated to the changes in wastewater physicochemical characteristics. The low degradation observed of SARS-CoV-2 in the spiked pellets were indicative of viral adhesion to wastewater solids, which correlated with changes in pH. Ultrasonication frequencies ranging from 4 to 16 kHz, increased SARS-CoV-2 concentrations in the supernatant between 3.30 and 35.65%, indicating viral RNA attachment to wastewater solids. These results highlight the importance of additional pretreatment methods for maximizing RNA recovery from wastewater samples. Based on these findings, it was deduced that wastewater preservation studies are essential, and pretreatment should be included in the WBE methodology.

Indexed as

SARS-CoV-2WastewaterBiomarkersCOVID-19HumansInfluenza A virusRNA, ViralTemperatureWastewater-Based Epidemiological MonitoringBiomarkersRNA, ViralWastewaterCOVID-19droplet digital PCRinfluenza ASARS-CoV-2wastewater-based epidemiology

Identifiers

PMID39007315

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.