Evidence map›Paper›PMID 39006446›Full record

ArticlemedRxiv : the preprint server for health sciences2024

MENSA, a Media Enriched with Newly Synthesized Antibodies, to Identify SARS-CoV-2 Persistence and Latent Viral Reactivation in Long-COVID.

Natalie S Haddad, Andrea Morrison-Porter, Hannah Quehl, Violeta Capric, Pedro A Lamothe, Fabliha Anam, Martin C Runnstrom, Alex D Truong, Adviteeya N Dixit, Matthew C Woodruff and 34 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in medRxiv : the preprint server for health sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

44 authors.

Natalie S HaddadDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.ORCID 0000-0002-1348-3094
Andrea Morrison-PorterDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Hannah QuehlDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Violeta CapricDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Pedro A LamotheDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Fabliha AnamDivision of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Martin C RunnstromDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Alex D TruongDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Adviteeya N DixitDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Matthew C WoodruffDivision of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Anting ChenDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Jiwon ParkDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Doan C NguyenDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Ian HentenaarDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Caroline Y KimDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Shuya KyuDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Brandon StewartDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Elizabeth WagmanDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Hannah GeoffroyDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Daniel SanzDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Kevin S CashmanDivision of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Richard P RamonellDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Monica Cabrera-MoraDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
David N AlterDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, 30322, USA.
John D RobackDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, 30322, USA.
Michael C HorwathDepartment of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, 30322, USA.
James B O'KeefeDivision of General Internal Medicine, Department of Medicine, Emory University School of Medicine, Atlanta, GA, 30322, USA.
Alexandra W DretlerMetro Infectious Disease Consultants, Decatur GA 30033, USA.
Ria GripaldoDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Samantha M YeligarDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Ted NatoliImmuneID, Inc Biotechnology Research, Waltham, MA, 02451, USA.
Viktoria BetinImmuneID, Inc Biotechnology Research, Waltham, MA, 02451, USA.
Rahulkumar PatelDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Kennedy VelaDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Mindy Rodriguez HernandezDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Sabeena UsmanDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
John VargheseDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Anum JalalDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
Saeyun LeeDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.ORCID 0000-0002-6133-5942
Sang N LeDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
R Toby AmossDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.
John L DaissMicroB-plex Inc, Atlanta, GA, 30332, USA.ORCID 0000-0002-9690-7701
Ignacio SanzDivision of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.ORCID 0000-0003-4182-587X
F Eun-Hyung LeeDivision of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.

Funding

Regulation of Pathogenic Plasma Cells in Human SLEP01AI125180 · NIAID · EMORY UNIVERSITY · PI Ignacio E. Sanz · 2016 to 2026
$28.2M
Virus-induced Cell Fate Decisions in Anti-Viral ImmunityU19AI109962 · NIAID · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI RANDALL, TROY D · 2014 to 2018
$11.6M
Project-004U54CA260563 · NCI · EMORY UNIVERSITY · PI DHODAPKAR, MADHAV V, SANZ, IGNACIO E. · 2020 to 2024
$10.2M
Training a New Generation of VaccinologistsT32AI074492 · NIAID · EMORY UNIVERSITY · PI Rama Rao Amara, Benjamin A Lopman · 2009 to 2026
$6.4M
Molecular Mechanisms of Human Long-lived Plasma Cell Generation in the Bone Marrow and SpleenU01AI141993 · NIAID · EMORY UNIVERSITY · PI Frances Eun-Hyung Lee · 2019 to 2026
$5.7M
Critical Care Training ProgramT32GM095442 · NIGMS · EMORY UNIVERSITY · PI Craig M Coopersmith · 2011 to 2026
$4.7M
The Emory Training Program in Lung HealthT32HL116271 · NHLBI · EMORY UNIVERSITY · PI BROWN, LOU ANN S, GUIDOT, DAVID MARSHALL · 2013 to 2022
$4.2M
Human Plasma Cell Maturation & Maintenance through CD138, TNFRSF, and Modulation of Ig SecretionR01AI172254 · NIAID · EMORY UNIVERSITY · PI Frances Eun-Hyung Lee · 2023 to 2026
$3.1M
Understanding Survival of Human Bone Marrow Long-Lived Plasma CellsR01AI121252 · NIAID · EMORY UNIVERSITY · PI LEE, FRANCES EUN-HYUNG · 2016 to 2020
$2.7M
Pathogenesis of RSV disease in the ElderlyU01AI045969 · NIAID · UNIVERSITY OF ROCHESTER · PI WALSH, EDWARD E · 2004 to 2008
$2.5M
NCI NIH HHS U54 CA260563NHLBI NIH HHS T32 HL116271NIAID NIH HHS P01 AI125180NIAID NIH HHS R01 AI121252NIAID NIH HHS R01 AI172254NIAID NIH HHS T32 AI074492NIAID NIH HHS U01 AI045969NIAID NIH HHS U01 AI141993NIAID NIH HHS U19 AI109962NIGMS NIH HHS T32 GM095442
6 · The paper itself

Abstract

Post-acute sequelae of SARS-CoV-2 (SARS2) infection (PASC) is a heterogeneous condition, but the main viral drivers are unknown. Here, we use MENSA, Media Enriched with Newly Synthesized Antibodies, secreted exclusively from circulating human plasmablasts, to provide an immune snapshot that defines the underlying viral triggers. We provide proof-of-concept testing that the MENSA technology can capture the new host immune response to accurately diagnose acute primary and breakthrough infections when known SARS2 virus or proteins are present. It is also positive after vaccination when spike proteins elicit an acute immune response. Applying the same principles for long-COVID patients, MENSA is positive for SARS2 in 40% of PASC vs none of the COVID recovered (CR) patients without any sequelae demonstrating ongoing SARS2 viral inflammation only in PASC. Additionally, in PASC patients, MENSAs are also positive for Epstein-Barr Virus (EBV) in 37%, Human Cytomegalovirus (CMV) in 23%, and herpes simplex virus 2 (HSV2) in 15% compared to 17%, 4%, and 4% in CR controls respectively. Combined, a total of 60% of PASC patients have a positive MENSA for SARS2, EBV, CMV, and/or HSV2. MENSA offers a unique antibody snapshot to reveal the underlying viral drivers in long-COVID thus demonstrating the persistence of SARS2 and reactivation of viral herpes in 60% of PASC patients.

Identifiers

PMID39006446
PMCPMC11245097

What OpenQuestion holds

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LicenceCC BY-ND
Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.