Evidence map›Paper›PMID 39005237›Full record

ArticleOne health (Amsterdam, Netherlands)2024

Metabarcoding using nanopore sequencing enables identification of diverse and zoonotic vector-borne pathogens from neglected regions: A case study investigating dogs from Bhutan.

Lucas G Huggins, Ugyen Namgyel, Pelden Wangchuk, Ushani Atapattu, Rebecca Traub, Vito Colella

Abstract read
In one paragraph

Article in One health (Amsterdam, Netherlands), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Genetic characterization of zoonoticMicrobiology spectrum · 2025
    Article
  5. Article
  6. Nanopore sequencing in veterinary medicine: from concepts to clinical applications.Frontiers in cellular and infection microbiology · 2025
    Review
  7. Characterization of co-infections of haemosporidian parasites in SwinhoeCurrent research in parasitology & vector-borne diseases · 2025
    Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Lucas G HugginsMelbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3050, Australia.
Ugyen NamgyelNational Centre for Animal Health, Serbithang, Thimphu 11001, Bhutan.
Pelden WangchukNational Centre for Animal Health, Serbithang, Thimphu 11001, Bhutan.
Ushani AtapattuMelbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3050, Australia.
Rebecca TraubMelbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3050, Australia.
Vito ColellaMelbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria 3050, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The diversity and prevalence of canine vector-borne pathogens (VBPs) in Bhutan have to date remained unexplored, whilst recent epidemiological surveys in other South Asian nations have found diseases caused by VBPs to be rife in local dog populations. Importantly, many of such VBPs can infect people as well, with a building body of evidence identifying potentially zoonotic rickettsial organisms infecting humans in Bhutan. Given the lack of data on canine pathogens in Bhutan we employed a suite of deep-sequencing metabarcoding methods using Oxford Nanopore Technologies' MinION™ device to holistically characterise the bacterial, apicomplexan and filarial worm blood-borne pathogens of dogs in the country's south. Of the 95 stray, owned and community dogs sampled 78% (95% CI = 69%-85%) were infected with at least one VBP. Pathogen species detected were highly diverse including the bacteria

Indexed as

BartonellaDirofilaria sp. hongkongensisEhrlichiaMinIONNext-generation sequencingOne HealthSetaria tundraZoonosis

Identifiers

PMID39005237
PMCPMC11238191

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.